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Surface Plasmon Resonance (SPR)

This optical method allows detection and characterization of the binding of biological molecules. In SPR-assays, one of the interactants is immobilized on the surface of a thin metallic film. When a binding partner is added, the refractive index of the film will be altered in a characteristic way, thereby allowing the time-resolved study of interaction between the two materials.

Total reflection: I = I0
attentuated total reflection: I < I0 (SPR)

  • Cukkemane A, Becker N, Zielinski M, Frieg B, Lakomek NA, Heise H, SchröderGF, Willbold D, Weiergräber OH 
    Conformational heterogeneity coupled with β-fibril formation of a scaffold protein involved in chronic mental illnesses 
    Transl Psychiatry 11, 639 (2021) 
  • López-Pérez A, Freischem S, Grimm I, Weiergräber O, Dingley AJ, López-AlbercaMP, Waldmann H, Vollmer W, Kumar K, Vuong C 
    Discovery of pyrrolidine-2,3-diones as novel inhibitors of P. aeruginosa PBP3 
    Antibiotics 10, 529 (2021) 
  • Cavini IA, Munte CE, Erlach MB, van Groen T, Kadish I, Zhang T, Ziehm T, Nagel-Steger L, Kutzsche J, Kremer W, Willbold D, Kalbitzer HR. 
    Inhibition of amyloid Aβ aggregation by high pressures or specific d-enantiomeric peptides. 
    Chem Commun (Camb). 54(26), 3294-3297 (2018) 
  • Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C 
    Biophysical insights from a single chain camelid antibody directed against the disrupted in schizophrenia 1 protein 
    PLoS One 13, e0191162 (2018) 
  • Anđela Šarić, Alexander K. Buell, Georg Meisl, Thomas C. T. Michaels, Sara Linse, Tuomas P. J. Knowles and Daan Frenkel 
    Physical determinants for the self-replication of protein fibrils 
    Nature Physics 12, 874-880 (2016) 
  • Ziehm T, Brener O, van Groen T, Kadish I, Frenzel D, Tusche M, Kutzsche J, Reiß K, Gremer L, Nagel-Steger L, Willbold D. 
    Increase of Positive Net Charge and Conformational Rigidity Enhances the Efficacy of d-Enantiomeric Peptides Designed to Eliminate Cytotoxic Aβ Species. 
    ACS Chem Neurosci. 7 (8), 1088–1096 (2016) 
  • Hung Y-F, Schwarten M, Schünke S, Thiagarajan-Rosenkranz P, Hoffmann S, Sklan EH, Willbold D, Koenig BW 
    Dengue virus NS4A cytoplasmic domain binding to liposomes is sensitive to membrane curvature 
    BBA - Biomembranes 1848, 1119-1126 (2015) 
  • Shaykhalishahi H, Mirecka EA, Gauhar A, Grüning CSR, Willbold D, Härd T, Stoldt M, Hoyer W 
    A beta-hairpin-binding protein for three different disease-related amyloidogenic proteins 
    ChemBioChem 16, 411-414 (2015) 
  • Frenzel D, Glück JM, Brener O, Oesterhelt F, Nagel-Steger L, Willbold D 
    Immobilization of homogeneous monomeric, oligomeric and fibrillar Aβ species for reliable SPR measurements 
    PLoS ONE 9, e89490 (2014) 
  • Frenzel D, Willbold D 
    Kinetic titration series with biolayer interferometry 
    PLoS ONE 9, e106882 (2014) 
  • Glück JM 
    Ligand interaction analysis of membrane-anchored proteins. 
    Schriften des Forschungszentrums Jülich Reihe Gesundheit, Band 31, (2011) 
  • Glück JM, Koenig BW, Willbold D 
    Nanodiscs allow the use of integral membrane proteins as analytes in surface plasmon resonance studies. 
    Anal. Biochem. 408, 46-52 (2011) 
  • Bartnik D, Funke SA, Andrei-Selmer L-C, Bacher M, Dodel R, Willbold D 
    Differently selected D-enantiomeric peptides act on different Aß species. 
    Rejuvenation Res. 13, 202-205 (2010) 
  • Hoffmann S, Funke SA, Wiesehan K, Moedder S, Glück JM, Feuerstein S, Gerdts M, Moetter J, Willbold D 
    Competitively selected protein ligands pay theirs increase in specificity by a decrease in affinity. 
    Mol. BioSyst 6, 116-123 (2010) 
  • Schwarten M, Mohrlüder J, Ma P, Stoldt M, Thielmann Y, Stangler T, Hersch N, Hoffmann B, Merkel R, Willbold D 
    Nix directly binds to GABARAP: a possible crosstalk between apoptosis and autophagy 
    Autophagy 5, 690-698 (2009) 
  • Elfrink K, Nagel-Steger L, Riesner D 
    Interaction of the cellular prion protein with raft-like lipid membranes. 
    Biol. Chem. 388, 79-90 (2007) 
    http://dx.doi.org/10.1515/BC.2007.010, 01/01/2007  
  • Mohrlüder J, Hoffmann Y, Stangler T, Hänel K, Willbold D 
    Identification of clathrin heavy chain as a direct interaction partner for the gamma-aminobutyric acid type A receptor associated protein 
    Biochemistry 46, 14537-14543 (2007) 
  • Mohrlüder J, Stangler T, Wiesehan K, Hoffmann Y, Mataruga A, Willbold D 
    Identification of calreticulin as a ligand of GABARAP by phage display screening of a peptide library 
    FEBS J 274, 5543-5555 (2007) 
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