Surface Plasmon Resonance (SPR)
This optical method allows detection and characterization of the binding of biological molecules. In SPR-assays, one of the interactants is immobilized on the surface of a thin metallic film. When a binding partner is added, the refractive index of the film will be altered in a characteristic way, thereby allowing the time-resolved study of interaction between the two materials.
Total reflection: I = I0
attentuated total reflection: I < I0 (SPR)
- Cukkemane A, Becker N, Zielinski M, Frieg B, Lakomek NA, Heise H, SchröderGF, Willbold D, Weiergräber OH
Conformational heterogeneity coupled with β-fibril formation of a scaffold protein involved in chronic mental illnesses
Transl Psychiatry 11, 639 (2021)
https://doi.org/10.1038/s41398-021-01765-1
- López-Pérez A, Freischem S, Grimm I, Weiergräber O, Dingley AJ, López-AlbercaMP, Waldmann H, Vollmer W, Kumar K, Vuong C
Discovery of Pyrrolidine-2,3-diones as Novel Inhibitors of P. aeruginosa PBP3
Antibiotics 10, 529 (2021)
https://doi.org/10.3390/antibiotics10050529
- Cavini IA, Munte CE, Erlach MB, van Groen T, Kadish I, Zhang T, Ziehm T, Nagel-Steger L, Kutzsche J, Kremer W, Willbold D, Kalbitzer HR.
Inhibition of amyloid Aβ aggregation by high pressures or specific d-enantiomeric peptides.
Chem Commun (Camb). 54(26), 3294-3297 (2018)
https://10.1039/c8cc01458b.
https://www.ncbi.nlm.nih.gov/pubmed/29537428
- Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C
Biophysical insights from a single chain camelid antibody directed against the disrupted in schizophrenia 1 protein
PLoS One 13, e0191162 (2018)
- Anđela Šarić, Alexander K. Buell, Georg Meisl, Thomas C. T. Michaels, Sara Linse, Tuomas P. J. Knowles and Daan Frenkel
Physical determinants for the self-replication of protein fibrils
Nature Physics 12, 874-880 (2016)
http://www.nature.com/nphys/journal/v12/n9/full/nphys3828.html
- Ziehm T, Brener O, van Groen T, Kadish I, Frenzel D, Tusche M, Kutzsche J, Reiß K, Gremer L, Nagel-Steger L, Willbold D.
Increase of Positive Net Charge and Conformational Rigidity Enhances the Efficacy of d-Enantiomeric Peptides Designed to Eliminate Cytotoxic Aβ Species.
ACS Chem Neurosci. 7 (8), 1088–1096 (2016)
http://pubs.acs.org/doi/abs/10.1021/acschemneuro.6b00047
- Hung Y-F, Schwarten M, Schünke S, Thiagarajan-Rosenkranz P, Hoffmann S, Sklan EH, Willbold D, Koenig BW
Dengue virus NS4A cytoplasmic domain binding to liposomes is sensitive to membrane curvature
BBA - Biomembranes 1848, 1119-1126 (2015)
- Shaykhalishahi H, Mirecka EA, Gauhar A, Grüning CSR, Willbold D, Härd T, Stoldt M, Hoyer W
A beta-hairpin-binding protein for three different disease-related amyloidogenic proteins
ChemBioChem 16, 411-414 (2015)
http://dx.doi.org/10.1002/cbic.201402552
- Frenzel D, Glück JM, Brener O, Oesterhelt F, Nagel-Steger L, Willbold D
Immobilization of homogeneous monomeric, oligomeric and fibrillar Aβ species for reliable SPR measurements
PLoS ONE 9, e89490 (2014)
- Frenzel D, Willbold D
Kinetic titration series with biolayer interferometry
PLoS ONE 9, e106882 (2014)
- Glück JM
Ligand interaction analysis of membrane-anchored proteins.
Schriften des Forschungszentrums Jülich Reihe Gesundheit, Band 31, (2011)
http://wwwzb1.fz-juelich.de/contentenrichment/inhaltsverzeichnisse/2010/verlag/gesund31_i.pdf
- Glück JM, Koenig BW, Willbold D
Nanodiscs allow the use of integral membrane proteins as analytes in surface plasmon resonance studies.
Anal. Biochem. 408, 46-52 (2011)
- Bartnik D, Funke SA, Andrei-Selmer L-C, Bacher M, Dodel R, Willbold D
Differently selected D-enantiomeric peptides act on different Aß species.
Rejuvenation Res. 13, 202-205 (2010)
- Hoffmann S, Funke SA, Wiesehan K, Moedder S, Glück JM, Feuerstein S, Gerdts M, Moetter J, Willbold D
Competitively selected protein ligands pay theirs increase in specificity by a decrease in affinity.
Mol. BioSyst 6, 116-123 (2010)
- Schwarten M, Mohrlüder J, Ma P, Stoldt M, Thielmann Y, Stangler T, Hersch N, Hoffmann B, Merkel R, Willbold D
Nix directly binds to GABARAP: a possible crosstalk between apoptosis and autophagy
Autophagy 5, 690-698 (2009)
- Elfrink K, Nagel-Steger L, Riesner D
Interaction of the cellular prion protein with raft-like lipid membranes.
Biol. Chem. 388, 79-90 (2007)
http://dx.doi.org/10.1515/BC.2007.010, 01/01/2007
- Mohrlüder J, Hoffmann Y, Stangler T, Hänel K, Willbold D
Identification of clathrin heavy chain as a direct interaction partner for the gamma-aminobutyric acid type A receptor associated protein
Biochemistry 46, 14537-14543 (2007)
- Mohrlüder J, Stangler T, Wiesehan K, Hoffmann Y, Mataruga A, Willbold D
Identification of calreticulin as a ligand of GABARAP by phage display screening of a peptide library
FEBS J 274, 5543-5555 (2007)