Autophagy
In recent years, autophagy has been recognized to be also essential for several functions of innate and acquired immunity, e.g. antigen presentation and elimination of cytosolic pathogens. Deregulation of autophagy has been implicated in a number of severe human diseases, in particular neurodegenerative disorders and neoplasias.
Scientists at the institute investigate the 
structural biology of cellular autophagy and the interaction of autophagy and viruses.
Further information
Autophagy
Publications
- Brown H, Chung M, Üffing A, Batistatou N, Tsang T, Doskocil S, Mao W, Willbold D, Bast RC Jr, Lu Z, Weiergräber OH, Kritzer JA
Structure-Based Design of Stapled Peptides That Bind GABARAP and Inhibit Autophagy
J Am Chem Soc 144, 14687-14697 (2022)
https://doi.org/10.1021/jacs.2c04699
- Klionsky DJ, et al, Schwarten M, et al, Weiergräber OH, et al, Willbold D, et al (many co-authors)
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
Autophagy 17, 1-382 (2021)
https://www.tandfonline.com/doi/full/10.1080/15548627.2020.1797280
- Dobner J, Simons IM, Rufinatscha K, Hänsch S, Schwarten M, Weiergräber OH, Abdollahzadeh I, Gensch T, Bode JG, Hoffmann S, Willbold D.
Deficiency of GABARAP but Not Its Paralogs Causes Enhanced EGF-induced EGFR Degradation
Cells 9, 1296 (2020)
https://doi.org/10.3390/cells9051296
https://www.mdpi.com/2073-4409/9/5/1296
- Sanwald JL, Dobner J, Simons IM, Poschmann G, Stühler K, Üffing A, Hoffmann S, Willbold D
Lack of GABARAP-Type Proteins Is Accompanied by Altered Golgi Morphology and Surfaceome Composition
Int J Mol Sci 22(1), 85 (2020)
https://doi.org/10.3390/ijms22010085
https://www.mdpi.com/1422-0067/22/1/85
- Sanwald JL, Poschmann G, Stühler K, Behrends C, Hoffmanns S, Willbold D.
The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles
Cells 9, 1468 (2020)
https://doi.org/10.3390/cells9061468
https://www.mdpi.com/2073-4409/9/6/1468
- Abdollahzadeh I, Hendriks J, Sanwald JL, Simons IM, Hoffmann S, Weiergräber OH, Willbold D, Gensch T
Autophagy-Related Proteins GABARAP and LC3B Label Structures of Similar Size but Different Shape in Super-Resolution Imaging
Molecules 24, E1833 (2019)
https://doi.org/10.3390/molecules24091833
- Krichel C, Möckel C, Schillinger O, Huesgen PF, Sticht H, Strodel B, Weiergräber OH, Willbold D, Neudecker P
Solution structure of the autophagy-related protein LC3C reveals a polyproline II motif on a mobile tether with phosphorylation site
Sci Rep 9, 14167 (2019)
https://doi.org/10.1038/s41598-019-48155-8
https://www.nature.com/articles/s41598-019-48155-8
- Schwarten M, Weiergräber OH, Petrović D, Strodel B, Willbold D
Structural Studies of Autophagy-Related Proteins
Methods Mol Biol 1880, 17-56 (2019)
https://link.springer.com/protocol/10.1007%2F978-1-4939-8873-0_2
- Simons IM, Mohrlüder J, Feederle R, Kremmer E, Zobel T, Dobner J, Bleffert N, Hoffmann S, Willbold D
The highly GABARAP specific rat monoclonal antibody 8H5 visualizes GABARAP in immunofluorescence imaging at endogenous levels
Sci Rep 9, 526 (2019)
https://doi.org/10.1038/s41598-018-36717-1
- Abdollahzadeh I, Schwarten M, Gensch T, Willbold D, Weiergräber OH
The Atg8 Family of Proteins—Modulating Shape and Functionality of Autophagic Membranes
Front Genet 8, 109 (2017)
https://doi.org/10.3389/fgene.2017.00109
- Weiergräber OH, Schwarten M, Strodel B, Willbold D.
Investigating Structure and Dynamics of Atg8 Family Proteins.
Methods Enzymol 587, 115-142 (2017)
- Klionsky DJ, et al, Schwarten M, et al, Willbold D, et al (many co-authors)
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Autophagy 12, 1-222 (2016)
http://www.tandfonline.com/doi/full/10.1080/15548627.2015.1100356
- Krichel C, Weiergräber OH, Pavlidou M, Mohrlüder J, Schwarten M, Willbold D, Neudecker P
Sequence-specific 1H, 15N, and 13C resonance assignments of the autophagy-related protein LC3C
Biomol NMR Assign 10, 41-43 (2016)
- Ma P, Schillinger O, Schwarten M, Lecher J, Hartmann R, Stoldt M, Mohrlüder J, Olubiyi O, Strodel B, Willbold D, Weiergräber OH
Conformational Polymorphism in Autophagy-Related Protein GATE-16
Biochemistry 54, 5469-5479 (2015)
- Michel M, Schwarten M, Decker C, Nagel-Steger L, Willbold D, Weiergräber OH
The mammalian autophagy initiator complex contains 2 HORMA domain proteins
Autophagy 11, 2300-2308 (2015)
http://dx.doi.org/10.1080/15548627.2015.1076605
- Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S, Mohrlüder J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergräber OH, Willbold D
Interaction of Bcl-2 with the autophagy-related GABAA receptor-associated protein (GABARAP): Biophysical characterization and functional implications
J Biol Chem 288, 37204-37215 (2013)
- Weiergräber OH, Mohrlüder J, Willbold D
Atg8 family proteins –- autophagy and beyond
in: Autophagy - A double-edged sword - Cell survival or death? ISBN 978-953-51-1062-0, Editor: Yannick Bailly (2013)
open access
- Klionsky DJ, et al, Willbold D, et al (many co-authors)
Guidelines for the use and interpretation of assays for monitoring autophagy.
Autophagy 8, 445-544 (2012)
- Ma P, Mohrlüder J, Schwarten M, Stoldt M, Singh SK, Hartmann R, Pacheco V, Willbold D
Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy
Chembiochem 11, 1967-1970 (2010)
- Pacheco V, Ma P, Thielmann Y, Hartmann R, Weiergräber OH, Mohrlüder J, Willbold D
Assessment of GABARAP self-association by its diffusion properties
J Biomol NMR 48, 49-58 (2010)
- Schwarten M, Stoldt M, Mohrlüder J, Willbold D
Solution structure of Atg8 reveals conformational polymorphism of the N-terminal domain
Biochem Biophys Res Commun 395, 426-431 (2010)
- Mohrlüder J, Schwarten M, Willbold D
Structure and potential function of gamma-aminobutyrate type A receptor-associated protein
FEBS J 276, 4989-5005 (2009)
- Schwarten M, Mohrlüder J, Ma P, Stoldt M, Thielmann Y, Stangler T, Hersch N, Hoffmann B, Merkel R, Willbold D
Nix directly binds to GABARAP: a possible crosstalk between apoptosis and autophagy
Autophagy 5, 690-698 (2009)
- Schwarten M, Stoldt M, Mohrlüder J, Willbold D
Sequence-specific 1H, 13C, and 15N resonance assignment of the autophagy-related protein Atg8
Biomol NMR Assign 3, 137-139 (2009)
- Thielmann Y, Weiergräber OH, Ma P, Schwarten M, Mohrlüder J, Willbold D
Comparative modeling of human NSF reveals a possible binding mode of GABARAP and GATE-16
Proteins 77, 637-646 (2009)
- Thielmann Y, Weiergräber OH, Mohrlüder J, Willbold D
Structural characterization of GABARAP-ligand interactions
Mol Biosyst 5, 575-579 (2009)
- Thielmann Y, Weiergräber OH, Mohrlüder J, Willbold D
Structural framework of the GABARAP-calreticulin interface - implications for substrate binding to endoplasmic reticulum chaperones
FEBS J 276, 1140-1152 (2009)
- Thielmann Y, Mohrlüder J, Koenig BW, Stangler T, Hartmann R, Becker K, Höltje HD, Willbold D
An indole binding site is a major determinant of the ligand specificity of the GABA type A receptor-associated protein GABARAP
Chembiochem 9, 1767-1775 (2008)
- Weiergräber OH, Stangler T, Thielmann Y, Mohrlüder J, Wiesehan K, Willbold D
Ligand binding mode of GABAA receptor-associated protein
J Mol Biol 381, 1320-1331 (2008)
- Mohrlüder J, Hoffmann Y, Stangler T, Hänel K, Willbold D
Identification of clathrin heavy chain as a direct interaction partner for the gamma-aminobutyric acid type A receptor associated protein
Biochemistry 46, 14537-14543 (2007)
- Mohrlüder J, Stangler T, Wiesehan K, Hoffmann Y, Mataruga A, Willbold D
Identification of calreticulin as a ligand of GABARAP by phage display screening of a peptide library
FEBS J 274, 5543-5555 (2007)
- Stangler T, Mayr LM, Willbold D
Solution structure of the GABAA receptor associated protein GABARAP: Implications for biological function and its regulation.
J. Biol. Chem. 277, 13363-13366 (2002)