Research Lakomek Group
Biophysical NMR Spectroscopy
Neurotransmitter release at the neuronal synapse is fundamental for signal transmission between nerve cells. Synaptic vesicles fuse with the pre-synaptic plasma membrane in a Ca2+ triggered and synchronized manner (neuronal exocytosis), to release neurotransmitters into the synaptic cleft. We study the structure and dynamics of membrane proteins and intrinsically disordered proteins that play a key role in neuronal exocytosis.
Proton-detected solid-state NMR spectroscopy for the structural investigation of membrane proteins
New nuclear magnetic resonance (NMR) based methods are developed to investigate the structure and dynamics of membrane proteins in a near-physiological lipid environment and at physiological temperatures. A focus is on novel proton-detected solid-state NMR experiments based on very fast magic angle spinning (MAS frequency between 60 and 110 kHz).
We develop new methods to investigate protein dynamics at different timescales, from pico- to milliseconds. Therefore, both solution and solid-state NMR methods are employed that provide complementary information. Here, the main focus is on intrinsically disordered proteins that interact with membranes.