SuFIDA (surfaced-based fluorescence immuno digital assay) is the combination of the reliable biochemical part of a sandwich ELISA and the readout using an imaging technique.
The SuFIDA process. A capture antibody immobilizes the analyte from the sample onto a glass surface. Components not bound to the surface are removed by washing. A fluorescence-labeled antibody is used as the detection probe, which recognizes an epitope on the target molecule that is not occupied by the capture antibody. Using highly sensitive fluorescence microscopy, all individual fluorescence-labeled probes on the surface can now be detected and virtually counted individually.
Eigenschaften von SuFIDA:
• Digital process
• Million readings per sample
• Multiplex measurement possible
• Linear measuring range over 4-5 orders of magnitude
• Femtomolar sensitivity
The core property of SuFIDA is the highly specific single molecule sensitivity. This is particularly important when, for example, body liquids like urine, saliva or blood contain only low abundant analytes. The SuFIDA Innovation Lab focuses on the development of biomarkers and analytes with high medical relevance and thus commercial potential.
For routine laboratory applications, fully automated microscopes are required, which are already available from numerous well-known manufacturers, for example for life cell imaging. It can be assumed that in a few years fully automated microscopes such as the In Cell Systems from GE Healthcare, but also equipment from other suppliers, will be part of the basic equipment of all modern laboratories and eventually also of commercial diagnostic laboratories.