Altendorf T, Gering I, Santiago-Schübel B, Aghabashlou Saisan S, Tamgüney G, Tusche M, Honold D, Schemmert S, Hoyer W, Mohrlüder J, Willbold D Stabilization of monomeric Tau protein by all D-enantiomeric peptide ligands as therapeutic strategy for Alzheimer's disease and other tauopathies Int J Mol Sci 24, 2161 (2023) https://doi.org/10.3390/ijms24032161
Kutzsche J, Guzman GA, Willuweit A, Kletke O, Wollert E, Gering I, Jürgens D, Breitkreutz J, Stark H, Beck-Sickinge AG, Klöcker N, Hidalgo P, Willbold D. An orally available N-type calcium channel inhibitor for the treatment of neuropathic pain. Br J Pharmacol. , (2023) https://pubmed.ncbi.nlm.nih.gov/38157867/
Kutzsche J, Schemmert S, Bujnicki T, Zafiu C, Halbgebauer S, Kraemer-Schulien V, Pils M, Blömeke L, Post J, Kulawik A, Jürgens D, Rossberg WM, Hümpel M, Bannach O, Otto M, Araujo JA, Willuweit A, Willbold D. Oral treatment with the all-d-peptide RD2 enhances cognition in aged beagle dogs - A model of sporadic Alzheimer's disease Heliyon 29;9(8):e18443, (2023) https://pubmed.ncbi.nlm.nih.gov/37609390/
Pils M, Dybala A, Rehn F, Blömeke L, Bujnicki T, Kraemer-Schulien V, Hoyer W, Riesner D, Willbold D, Bannach O Development and implementation of an internal quality control sample to standardize oligomer-based diagnostics of Alzheimer's disease Diagnostics 13, 1702 (2023) https://doi.org/10.3390/diagnostics13101702
Willuweit A, Humpert S, Schöneck M, Endepols H, Burda N, Gremer L, Gering I, Kutzsche J, Shah NJ, Langen KJ, Neumaier B, Willbold D, Drzezga A. Evaluation of the 18F-labeled analog of the therapeutic all-D-enantiomeric peptide RD2 for amyloid β imaging Eur J Pharm Sci 184:106421, (2023) https://doi.org/10.1016/j.ejps.2023.106421 https://pubmed.ncbi.nlm.nih.gov/36889654/
Bartl, J. and Zanini, M. and Bernardi, F. and Forget, A. and Blümel, L. and Talbot, J. and Picard D, Qin N, Cancila G, Gao Q, Nath S, Koumba IM, Wolter M, Kuonen F, Langini M, Beez, T, Munoz C, Pauck D, Marquardt V, Yu H, Souphron J, Korsch M, Mölders C, Berger D, Göbbels S, Meyer FD, Scheffler B, Rotblat B, Diederichs S, Ramaswamy V, Suzuki H, Oro A, Stühler K, Stefanski A, Fischer U, Leprivier G, Willbold D, Steger G, Buell A, Kool M, Lichter P, Pfister SM, Northcott PA, Taylor MD, Borkhardt A, Reifenberger G, Ayrault O, Remke M The HHIP-AS1 lncRNA promotes tumorigenicity through stabilization of dynein complex 1 in human SHH-driven tumors. Nat. Commun . 13, 4061 (2022) https://doi.org/10.1038/s41467-022-31574-z
Braczynski AK, Sevenich M, Gering I, Kupreichyk T, Agerschou ED, Kronimus Y, Habib P, Stoldt M, Willbold D, Schulz JB, Bach JP, Falkenburger BH, Hoyer W Alpha-synuclein-specific naturally occurring antibodies inhibit aggregation in vitro and in vivo Biomolecules 12, 469 (2022) https://doi.org/10.3390/biom12030469
Brown H, Chung M, Üffing A, Batistatou N, Tsang T, Doskocil S, Mao W, Willbold D, Bast RC Jr, Lu Z, Weiergräber OH, Kritzer JA Structure-Based Design of Stapled Peptides That Bind GABARAP and Inhibit Autophagy J Am Chem Soc 144, 14687-14697 (2022) https://doi.org/10.1021/jacs.2c04699
Hemmer S, Schulte M, Knieps-Grünhagen E, Granzin J, Willbold D, Jaeger KE, Batra-Safferling R, Panwalkar V, Krauss U. Residue alterations within a conserved hydrophobic pocket influence light, oxygen, voltage photoreceptor dark recovery. Photochem Photobiol Sci , (2022) https://doi.org/10.1007/s43630-022-00346-5
Kass B, Schemmert S, Zafiu C, Pils M, Bannach O, Kutzsche J, Bujnicki T, Willbold D. Aβ oligomer concentration in mouse and human brain and its drug-induced reduction ex vivo. Cell Rep Med. 3(5):100630, (2022) https://doi.org/10.1016/j.xcrm.2022.100630
Sevenich M, Honold D, Willuweit A, Kutzsche J, Mohrlüder J, Willbold D. Development of an α-synuclein fibril and oligomer specific tracer for diagnosis of Parkinson's disease, dementia with Lewy bodies and multiple system atrophy. Neurochem Int. 161, 105422 (2022) https://doi.org/10.1016/j.neuint.2022.105422 https://pubmed.ncbi.nlm.nih.gov/36252819/
Törner R, Kupreichyk T, Gremer L, Debled EC, Fenel D, Schemmert S, Gans P, Willbold D, Schoehn G, Hoyer W, Boisbouvier J Structural basis for the inhibition of IAPP fibril formation by the co-chaperonin prefoldin Nat Commun 13, 2363 (2022) https://doi.org/10.1038/s41467-022-30042-y
Weiergräber OH, Petrović D, Kislat A, Pattky M, Fabig J, Batra-Safferling R, Schulte am Esch J, Hänel K, Huhn C, Strodel B, Homey B, Willbold D Structure and Dynamics of Human Chemokine CCL16—Implications for Biological Activity Biomolecules 12, 1588 (2022) https://doi.org/10.3390/biom12111588
Arinkin V, Granzin J, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R. Structural determinants underlying the adduct lifetime in the LOV proteins of Pseudomonas putida. FEBS J 288(16), 4955-4972 (2021) https://doi.org/10.1111/febs.15785
Camargo LC, Honold D, Bauer R, Shah NJ, Langen KJ, Willbold D, Kutzsche J, Willuweit A, Schemmert S. Sex-Related Motor Deficits in the Tau-P301L Mouse Model Biomedicines 9(9):1160, (2021) https://pubmed.ncbi.nlm.nih.gov/34572348/
Camargo LC, Schöneck M, Sangarapillai N, Honold D, Shah NJ, Langen KJ, Willbold D, Kutzsche J, Schemmert S, Willuweit A PEAβ Triggers Cognitive Decline and Amyloid Burden in a Novel Mouse Model of Alzheimer's Disease Int J Mol Sci. 22(13):706, (2021) https://pubmed.ncbi.nlm.nih.gov/34209113/
Cukkemane A, Becker N, Zielinski M, Frieg B, Lakomek NA, Heise H, SchröderGF, Willbold D, Weiergräber OH Conformational heterogeneity coupled with β-fibril formation of a scaffold protein involved in chronic mental illnesses Transl Psychiatry 11, 639 (2021) https://doi.org/10.1038/s41398-021-01765-1
Freischem S, Grimm I, López-Pérez A, Willbold D, Klenke B, Vuong C, DingleyAJ, Weiergräber OH Interaction Mode of the Novel Monobactam AIC499 Targeting Penicillin Binding Protein 3 of Gram-Negative Bacteria Biomolecules 11, 1057 (2021) https://doi.org/10.3390/biom11071057
Klionsky DJ, et al, Schwarten M, et al, Weiergräber OH, et al, Willbold D, et al (many co-authors) Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) Autophagy 17, 1-382 (2021) https://www.tandfonline.com/doi/full/10.1080/15548627.2020.1797280
König AS, Rösener NS, Gremer L, Tusche M, Flender D, Reinartz E, Hoyer W, Neudecker P, Willbold D, Heise H Structural details of amyloid β oligomers in complex with human prion protein as revealed by solid-state MAS NMR spectroscopy J Biol Chem 296, 100499 (2021) https://doi.org/10.1016/j.jbc.2021.100499
Post J, Kogel V, Schaffrath A, Lohmann P, Shah NJ, Langen K-J, Willbold D, Willuweit A, Kutzsche J A Novel Anti-Inflammatory d-Peptide Inhibits Disease Phenotype Progression in an ALS Mouse Model. Molecules 26(6):1590, (2021) https://doi.org/10.3390/molecules26061590
Post J, Schaffrath A, Gering I, Hartwig S, Lehr S, Shah NJ, Langen KJ, Willbold D, Kutzsche J, Willuweit A. Oral Treatment with RD2RD2 Impedes Development of Motoric Phenotype and Delays Symptom Onset in SOD1 G93A Transgenic Mice Int J Mol Sci . 22(13):7066, (2021) https://pubmed.ncbi.nlm.nih.gov/34209129/
Schemmert S, Camargo LC, Honold D, Gering I, Kutzsche J, Willuweit A, Willbold D n Vitro and In Vivo Efficacies of the Linear and the Cyclic Version of an All-d-Enantiomeric Peptide Developed for the Treatment of Alzheimer's Disease Int J Mol Sci. 22(12):6553, (2021) https://pubmed.ncbi.nlm.nih.gov/34207233/
Willbold D, Strodel B, Schröder GF, Hoyer W, Heise H Amyloid-type protein aggregation and prion-like properties of amyloids Chem Rev 121, 8285-8307 (2021) https://doi.org/10.1021/acs.chemrev.1c00196
Dobner J, Simons IM, Rufinatscha K, Hänsch S, Schwarten M, Weiergräber OH, Abdollahzadeh I, Gensch T, Bode JG, Hoffmann S, Willbold D. Deficiency of GABARAP but Not Its Paralogs Causes Enhanced EGF-induced EGFR Degradation Cells 9, 1296 (2020) https://doi.org/10.3390/cells9051296 https://www.mdpi.com/2073-4409/9/5/1296
Kutzsche J, Jürgens D, Willuweit A, Adermann K, Fuchs C, Simons S, Windisch M, Hümpel M, Rossberg W, Wolzt M, Willbold D. Safety and Pharmacokinetics of the Orally Available Antiprionic Compound PRI-002: A Single and Multiple Ascending Dose Phase I Study Alzheimers Dement (N Y) , 20;6(1):e12001 (2020) https://pubmed.ncbi.nlm.nih.gov/32211506/
Abdollahzadeh I, Hendriks J, Sanwald JL, Simons IM, Hoffmann S, Weiergräber OH, Willbold D, Gensch T Autophagy-Related Proteins GABARAP and LC3B Label Structures of Similar Size but Different Shape in Super-Resolution Imaging Molecules 24, E1833 (2019) https://doi.org/10.3390/molecules24091833
Agerschou ED, Flagmeier P, Saridaki T, Galvagnion C, Komnig D, Heid L, Prasad V, Shaykhalishahi H, Willbold D, Dobson CM, Voigt A, Falkenburger B, Hoyer W, Buell AK An engineered monomer binding-protein for α-synuclein efficiently inhibits the proliferation of amyloid fibrils eLife 8, e46112 (2019) https://doi.org/10.7554/eLife.46112
Elfgen A, Hupert M, Bochinsky K, Tusche M, González de San Román Martin E, Gering I, Sacchi S, Pollegioni L, Huesgen PF, Hartmann R, Santiago-Schübel B, Kutzsche J, Willbold D. Metabolic resistance of the D-peptide RD2 developed for direct elimination of amyloid-β oligomers Sci Rep 9(1), 5715 (2019) http://10.1038/s41598-019-41993-6 https://www.nature.com/articles/s41598-019-41993-6
Klinker S, Stindt S, Gremer L, Bode JG, Gertzen CGW, Gohlke H, Weiergräber OH, Hoffmann S, Willbold D Phosphorylated tyrosine 93 of hepatitis C virus nonstructural protein 5A is essential for interaction with host c-Src and efficient viral replication J Biol Chem 294, 7388-7402 (2019) https://doi.org/10.1074/jbc.RA119.007656
Schemmert S, Schartmann E, Honold D, Zafiu C, Ziehm T, Langen KJ, Shah NJ, Kutzsche J, Willuweit A, Willbold D. Deceleration of the neurodegenerative phenotype in pyroglutamate-Aβ accumulating transgenic mice by oral treatment with the Aβ oligomer eliminating compound RD2. Neurobiol Dis. 124, 36-45 (2019) https://www.sciencedirect.com/science/article/pii/S0969996118307320
Schemmert S, Schartmann E, Zafiu C, Kass B, Hartwig S, Lehr S, Bannach O, Langen KJ, Shah NJ, Kutzsche J, Willuweit A, Willbold D. Aβ Oligomer Elimination Restores Cognition in Transgenic Alzheimer's Mice with Full-blown Pathology. Mol Neurobiol. 56(3):, 2211-2223 (2019) https://10.1007/s12035-018-1209-3 https://www.ncbi.nlm.nih.gov/pubmed/30003517
Simons IM, Mohrlüder J, Feederle R, Kremmer E, Zobel T, Dobner J, Bleffert N, Hoffmann S, Willbold D The highly GABARAP specific rat monoclonal antibody 8H5 visualizes GABARAP in immunofluorescence imaging at endogenous levels Sci Rep 9, 526 (2019) https://doi.org/10.1038/s41598-018-36717-1
Caruso IP, Panwalkar V, Coronado MA, Dingley AJ, Cornélio ML, Willbold D, Arni RK, Eberle RJ Structure and interaction of Corynebacterium pseudotuberculosis cold shock protein A with Y-box single-stranded DNA fragment FEBS J 285, 372-390 (2018) https://doi.org/10.1111/febs.14350
Cavini IA, Munte CE, Erlach MB, van Groen T, Kadish I, Zhang T, Ziehm T, Nagel-Steger L, Kutzsche J, Kremer W, Willbold D, Kalbitzer HR. Inhibition of amyloid Aβ aggregation by high pressures or specific d-enantiomeric peptides. Chem Commun (Camb). 54(26), 3294-3297 (2018) https://10.1039/c8cc01458b. https://www.ncbi.nlm.nih.gov/pubmed/29537428
Coronado MA, Eberle RJ, Bleffert N, Feuerstein S, Olivier DS, de Moraes FR, Willbold D, Arni RK Zika virus NS2B/NS3 proteinase: A new target for an old drug - Suramin a lead compound for NS2B/NS3 proteinase inhibition. Antiviral Res. 160, 118-125 (2018)
Dunkelmann T, Schemmert S, Honold D, Teichmann K, Butzküven E, Demuth HU, Shah NJ, Langen KJ, Kutzsche J, Willbold D, Willuweit A. Comprehensive Characterization of the Pyroglutamate Amyloid-β Induced Motor Neurodegenerative Phenotype of TBA2.1 Mice. J Alzheimers Dis. 63(1), 115-130 (2018) https://10.3233/JAD-170775 https://www.ncbi.nlm.nih.gov/pubmed/29578479
Fettweiss T, Röllen K, Granzin J, Reiners O, Endres S, Drepper T, Willbold D, Jaeger KE, Batra-Safferling R*, Krauss U* Mechanistic Basis of the Fast Dark Recovery of the Short LOV Protein DsLOV from Dinoroseobacter shibae. Biochemistry 57, 4833–4847 (2018) http://dx.doi.org/10.1021/acs.biochem.8b00645
Hasecke F, Miti T, Perez C, Barton J, Schölzel D, Gremer L, Grüning CSR, Matthews G, Meisl G, Knowles TPJ, Willbold D, Neudecker P, Heise H, Ullah G, Hoyer W, Muschol M Origin of metastable oligomers and their effects on amyloid fibril self-assembly Chem. Sci. 9, 5937-5948 (2018) https://doi.org/10.1039/c8sc01479e
Rösener NS, Gremer L, Reinartz E, König A, Brener O, Heise H, Hoyer W, Neudecker P, Willbold D A d-enantiomeric peptide interferes with heteroassociation of amyloid-β oligomers and prion protein J. Biol. Chem. 293, 15748-15764 (2018) https://doi.org/10.1074/jbc.RA118.003116
Schartmann E, Schemmert S, Niemietz N, Honold D, Ziehm T, Tusche M, Elfgen A, Gering I, Brener O, Shah NJ, Langen KJ, Kutzsche J, Willbold D, Willuweit A. In Vitro Potency and Preclinical Pharmacokinetic Comparison of All-D-Enantiomeric Peptides Developed for the Treatment of Alzheimer's Disease. J Alzheimers Dis 64(3), 859-873 (2018) https://10.3233/JAD-180165 https://www.ncbi.nlm.nih.gov/pubmed/29966196
Schulte M, Panwalkar V, Freischem S, Willbold D, Dingley, AJ Proline restricts loop 1 conformation of the high affinity WW domain from human Nedd4-1 to a ligand binding-competent type 1 beta-turn J Phys Chem B 122, 4219-4230 (2018) https://doi.org/10.1021/acs.jpcb.7b11637
Schulte M, Petrović D, Neudecker P, Hartmann R, Pietruszka J, Willbold S, Willbold D, Panwalkar V Conformational sampling of the intrinsically disordered C-terminal tail of DERA is important for enzyme catalysis ACS Catal 8, 3971-3984 (2018) https://doi.org/10.1021/acscatal.7b04408
Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C Biophysical insights from a single chain camelid antibody directed against the disrupted in schizophrenia 1 protein PLoS One 13, e0191162 (2018)
Abdollahzadeh I, Schwarten M, Gensch T, Willbold D, Weiergräber OH The Atg8 Family of Proteins—Modulating Shape and Functionality of Autophagic Membranes Front Genet 8, 109 (2017) https://doi.org/10.3389/fgene.2017.00109
Arinkin V, Granzin J, Röllen K, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R. Structure of a LOV protein in apo-state and implications for construction of LOV-based optical tools Scientific Reports 7(42971), (2017) http://dx.doi.org/10.1038/srep42971
Boeske A, Schwarten M, Ma P, Tusche M, Mötter J, Möller C, Neudecker P, Hoffmann S, Willbold D Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization Scientific Reports 7, 5979 (2017)
Caruso IP, Panwalkar V, Coronado MA, Dingley AJ, Cornélio ML, Willbold D, Arni RK, Eberle RJ. NMR studies of cold shock protein A from Corynebacterium pseudotuberculosis: structure and interaction with Y-box single-stranded DNA fragment. FEBS J. 285, 372-390 (2017)
Dammers C, Reiss K, Gremer L, Lecher J, Ziehm T, Stoldt M, Schwarten M, Willbold D. Pyroglutamate-modified amyloid-β(3-42) shows α-helical intermediates before amyloid formation. Biophys. J. 112, 1621-1633 (2017)
Dammers C, Schwarten M, Buell AK, Willbold D Pyroglutamate-modified Aβ(3-42) affects aggregation kinetics of Aβ(1-42) by accelerating primary and secondary pathways Chem. Sci. 8, 4996-5004 (2017) http://pubs.rsc.org/en/content/articlehtml/2017/sc/c6sc04797a
Gushchin I, Melnikov I, Polovinkin V, Ishchenko A, Yuzhakova A, Buslaev P, Bourenkov G, Grudinin S, Round E, Balandin T, Borshchevskiy V, Willbold D, Leonard G, Büldt G, Popov A, Gordeliy V. Mechanism of transmembrane signaling by sensor histidine kinases. Science 356, eaah6345 (2017)
Klein AN, Ziehm T, van Groen T, Kadish I, Elfgen A, Tusche M, Thomaier M, Reiss K, Brener O, Gremer L, Kutzsche J, Willbold D Optimization of D-peptides for Aβ monomer binding specificity enhances their potential to eliminate toxic Aβ oligomers ACS Chem Neurosci 8, 1889-1900 (2017)
Kühbach K, Hülsemann M, Herrmann Y, Kravchenko K, Kulawik A, Linnartz C, Peters L, Wang K, Willbold J, Willbold D, Bannach O. Application of an amyloid beta oligomer standard in the sFIDA assay. In: Biomarkers of Alzheimer's Disease: The Present and the Future. Eds. Sylvain Lehmann, Charlotte Elisabeth Teunissen. , 39-44 (2017) ISBN 9782889450411
Kutzsche J, Schemmert S, Tusche M, Neddens J, Rabl R, Jürgens D, Brener O, Willuweit A, Hutter-Paier B, Willbold D. Large-Scale Oral Treatment Study with the Four Most Promising D3-Derivatives for the Treatment of Alzheimer's Disease. Molecules 22(10), (2017) http://10.3390/molecules22101693 https://www.ncbi.nlm.nih.gov/pubmed/28994710
Nikolaev M, Round E, Gushchin I, Polovinkin V, Balandin T, Kuzmichev P, Shevchenko V, Borshchevskiy V, Kuklin A, Round A, Bernhard F, Willbold D, Büldt G, Gordeliy V. Integral membrane proteins can be crystallized directly from nanodiscs. Cryst. Growth Des. 17, 945-948 (2017)
Schartmann E, Schemmert S, Ziehm T, Leithold LHE, Jiang N, Tusche M, Shah NJ, Langen KJ, Kutzsche J, Willbold D, Willuweit A. Comparison of blood-brain barrier penetration efficiencies between linear and cyclic all-d-enantiomeric peptides developed for the treatment of Alzheimer's disease. Eur J Pharm Sci. 17, 0928-0987 (2017) https://doi.org/10.1016/j.ejps.2017.12.005 https://www.ncbi.nlm.nih.gov/pubmed/29225107
van Groen T, Schemmert S, Brener O, Gremer L, Ziehm T, Tusche M, Nagel-Steger L, Kadish I, Schartmann E, Elfgen A, Jürgens D, Willuweit A, Kutzsche J, Willbold D. The Aβ oligomer eliminating D-enantiomeric peptide RD2 improves cognition without changing plaque pathology. Sci Rep. 7, 16275 (2017) https://doi.org/10.1038/s41598-017-16565-1 https://www.nature.com/articles/s41598-017-16565-1
Vasudevan JAA, Hofmann H, Willbold D, Häussinger D, Koenig BW, Münk C. Enhancing the catalytic deamination activity of APOBEC3C is insufficient to inhibit Vif-deficient HIV-1. J. Mol. Biol. 429, 1171-1191 (2017)
Volkov O, Kovalev K, Polovinkin V, Borshchevskiy V, Bamann C, Astashkin R, Marin E, Popov A, Balandin T, Willbold D, Büldt G, Bamberg E, Gordeliy V Structural insights into ion conduction by channelrhodopsin 2 Science 358, eaan8862, (2017)
Weiergräber OH, Schwarten M, Strodel B, Willbold D. Investigating Structure and Dynamics of Atg8 Family Proteins. Methods Enzymol 587, 115-142 (2017)
Wolff M, Zhang-Haagen B, Decker C, Barz B, Schneider M, Biehl R, Radulescu A, Strodel B, Willbold D, Nagel-Steger L Aβ42 pentamers/hexamers are the smallest detectable oligomers in solution Sci Rep 7, 2493 (2017) http://dx.doi.org/10.1038/s41598-017-02370-3
Yerabham ASK, Mas PJ, Decker C, Soares DC, Weiergräber OH, Nagel-Steger L, Willbold D, Hart DJ, Bradshaw NJ, Korth C. A structural organization for Disrupted in Schizophrenia 1 protein, identified by high-throughput screening, reveals distinctly folded regions which are bisected by mental illness related mutations. J Biol Chem 292, 6468-6477 (2017) http://dx.doi.org/10.1074/jbc.M116.773903
Dammers C, Yolcu D, Kukuk L, Willbold D, Pickhardt M, Mandelkow E, Horn AH, Sticht H, Malhis MN, Will N, Schuster J, Funke SA. Selection and characterization of Tau binding D-enantiomeric peptides with potential for therapy of Alzheimer disease PLoS One 11, e0167432 , (2016) http://dx.doi.org/10.1371/journal.pone.0167432
Dick M, Hartmann R, Weiergräber OH, Bisterfeld C, Classen T, Schwarten M, Neudecker P, Willbold D, Pietruszka J. Mechanism-based inhibition of an aldolase at high concentrations of its natural substrate acetaldehyde: structural insights and protective strategies. Chem. Sci. 7, 4492-4502 (2016)
Donner L, Fälker K, Gremer L, Klinker S, Pagani G, Ljungberg LU, Lothmann K,Rizzi F, Schaller M, Gohlke H, Willbold D, Grenegard M, Elvers M. Platelets contribute to amyloid-β aggregation in cerebral vessels through integrin αIIbβ3-induced outside-in signaling and clusterin release Sci Signal 9(429), ra52 (2016) http://10.1126/scisignal.aaf6240 http://stke.sciencemag.org/content/9/429/ra52.long
Jiang N, Frenzel D, Schartmann E, van Groen T, Kadish I, Shah NJ, Langen KJ, Willbold D, Willuweit A. Blood-brain barrier penetration of an Aβ-targeted, arginine-rich, D-enantiomeric peptide BBA Biomembranes 1858, 2717-2724 (2016)
Klein AN, Ziehm T, Tusche M, Buitenhuis J, Bartnik D, Boeddrich A, Wiglenda T, Wanker E, Funke SA, Brener O, Gremer L, Kutzsche J, Willbold D. Optimization of the All-D Peptide D3 for Aβ Oligomer Elimination. PLoS One 11(4), (2016) http://dx.doi.org/10.1371/journal.pone.0153035 Erratum in: PLoS One. 2016;11(7):e0158960. http://dx.doi.org/10.1371/journal.pone.0158960
Klionsky DJ, et al, Schwarten M, et al, Willbold D, et al (many co-authors) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12, 1-222 (2016) http://www.tandfonline.com/doi/full/10.1080/15548627.2015.1100356
Koch K, Hartmann R, Schröter F, Suwala AK, Maciaczyk D, Krüger AC, Willbold D, Kahlert UD, Maciaczyk J. Reciprocal regulation of the cholinic phenotype and epithelial-mesenchymal transition in glioblastoma cells. Oncotarget , (2016)
Krichel C, Weiergräber OH, Pavlidou M, Mohrlüder J, Schwarten M, Willbold D, Neudecker P Sequence-specific 1H, 15N, and 13C resonance assignments of the autophagy-related protein LC3C Biomol NMR Assign 10, 41-43 (2016)
Leithold LH, Jiang N, Post J, Niemietz N, Schartmann E, Ziehm T, Kutzsche J, Shah NJ, Breitkreutz J, Langen KJ, Willuweit A, Willbold D. Pharmacokinetic properties of tandem D-peptides designed for treatment of Alzheimer's disease Eur. J. Pharm. Sci. 89, 31-38 (2016) http://dx.doi.org/10.1016/j.ejps.2016.04.016
Leithold LH, Jiang N, Post J, Ziehm T, Schartmann E, Kutzsche J, Shah NJ, Breitkreutz J, Langen KJ, Willuweit A, Willbold D. Pharmacokinetic Properties of a Novel D-Peptide Developed to be Therapeutically Active Against Toxic β-Amyloid Oligomers Pharm Res. 33, 328-336 (2016)
Panwalkar V, Neudecker P, Schmitz M, Lecher J, Schulte M, Medini K, Stoldt M, Brimble MA, Willbold D, Dingley AJ. The Nedd4-1 WW Domain Recognizes the PY Motif Peptide through Coupled Folding and Binding Equilibria Biochemistry 55, 659-674 (2016) http://pubs.acs.org/doi/full/10.1021/acs.biochem.5b01028
Wang W, Ma P, Dong H, Krause HJ, Zhang Y, Willbold D, Offenhaeusser A, Gu Z A. A magnetic nanoparticles relaxation sensor for protein-protein interaction detection at ultra-low magnetic field. Biosens. Bioelectron. 80, 661-665 (2016) http://dx.doi.org/10.1016/j.bios.2016.02.037
Zhang-Haagen B, Biehl R, Nagel-Steger L, Radulescu A, Richter D, Willbold D. Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering. PLoS One 11(2), e0150267 (2016) http://dx.doi.org/10.1371/journal.pone.0150267
Ziehm T, Brener O, van Groen T, Kadish I, Frenzel D, Tusche M, Kutzsche J, Reiß K, Gremer L, Nagel-Steger L, Willbold D. Increase of Positive Net Charge and Conformational Rigidity Enhances the Efficacy of d-Enantiomeric Peptides Designed to Eliminate Cytotoxic Aβ Species. ACS Chem Neurosci. 7 (8), 1088–1096 (2016) http://pubs.acs.org/doi/abs/10.1021/acschemneuro.6b00047
Börger C, Schünke S, Lecher J, Stoldt M, Winkhaus F, Kaupp UB, Willbold D Resonance assignment of the ligand-free cyclic nucleotide-binding domain from the murine ion channel HCN2 Biomol. NMR Assign. 9, 243-246 (2015)
Brener O, Dunkelmann T, Gremer L, van Groen T, Mirecka EA, Kadish I, Willuweit A, Kutzsche J, Jürgens D, Rudolph S, Tusche M, Bongen P, Pietruszka J, Oesterhelt F, Langen K-J, Demuth H-U, Janssen A, Hoyer W, Funke SA, Nagel-Steger L, Willbold D QIAD assay for quantitating a compound's efficacy in elimination of toxic Aβ oligomers. Sci Rep 5, 13222 (2015) http://dx.doi.org/10.1038/srep13222
Dammers C, Gremer L, Neudecker P, Demuth HU, Schwarten M, Willbold D Purification and Characterization of Recombinant N-Terminally Pyroglutamate-Modified Amyloid-β Variants and Structural Analysis by Solution NMR Spectroscopy PLoS One 10, e0139710 (2015)
Dammers C, Gremer L, Reiß K, Klein AN, Neudecker P, Hartmann R, Sun N, Demuth HU, Schwarten M, Willbold D Structural Analysis and Aggregation Propensity of Pyroglutamate Aβ(3-40) in Aqueous Trifluoroethanol PLoS One 10, e0143647 (2015)
Endres S, Granzin J, Circolone F, Stadler A, Krauss U, Drepper T, Svensson V, Knieps-Grünhagen E, Wirtz A, Cousin A, Tielen P, Willbold D, Jaeger K-E, Batra-Safferling R Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae. BMC Microbiology 15, 30 (2015) http://www.biomedcentral.com/1471-2180/15/30
Goossens K, Prior M, Pacheco V, Willbold D, Müllen K, Enderlein J, Hofkens J, Gregor I Accurate diffusion coefficients of organosoluble reference dyes in organic media measured by dual-focus fluorescence correlation spectroscopy. ACS Nano 9, 7360-7373 (2015)
Gushchin I, Shevchenko V, Polovinkin V, Kovalev K, Alekseev A, Round E, Borshchevskiy V, Balandin T, Popov A, Gensch T, Fahlke C, Bamann C, Willbold D, Büldt G, Bamberg E, Gordeliy V Crystal structure of a light-driven sodium pump. Nat. Struct. Mol. Biol. 22, 390-395 (2015)
Hung Y-F, Schwarten M, Hoffmann S, Willbold D, Sklan EH, Koenig BW Amino Terminal Region of Dengue Virus NS4A Cytosolic Domain Binds to Highly Curved Liposomes Viruses 7, 4119-4130 (2015) Link
Hung Y-F, Schwarten M, Schünke S, Thiagarajan-Rosenkranz P, Hoffmann S, Sklan EH, Willbold D, Koenig BW Dengue virus NS4A cytoplasmic domain binding to liposomes is sensitive to membrane curvature BBA - Biomembranes 1848, 1119-1126 (2015)
Jiang N, Leithold LH, Post J, Ziehm T, Mauler J, Gremer L, Cremer M, Schartmann E, Shah NJ, Kutzsche J, Langen KJ, Breitkreutz J, Willbold D, Willuweit A Preclinical Pharmacokinetic Studies of the Tritium Labelled D-Enantiomeric Peptide D3 Developed for the Treatment of Alzheimer´s Disease PLoS One 10, e0128553 (2015)
Kahlert UD, Koch K, Hartmann R, Suwala AK, Cheng M, Maciaczyk D, Eberhart CG, Willbold D, Glunde K, Maciaczyk J The effect of neurosphere culture conditions on cellular metabolism of glioma cells. Folia Neuropathologica 53, 219-225 (2015)
Ma P, Schillinger O, Schwarten M, Lecher J, Hartmann R, Stoldt M, Mohrlüder J, Olubiyi O, Strodel B, Willbold D, Weiergräber OH Conformational Polymorphism in Autophagy-Related Protein GATE-16 Biochemistry 54, 5469-5479 (2015)
Ma P, Xue Y, Coquelle N, Haller JD, Yuwen T, Ayala I, Mikhailovskii O, Willbold D, Colletier J-P, Skrynnikov NR, Schanda P Observing the overall rocking motion of a protein in a crystal. Nat. Commun. 6, 8361 (2015)
Mandler M, Santic R, Gruber P, Cinar Y, Pichler D, Funke SA, Willbold D, Schneeberger A, Schmidt W, Mattner F Tailoring the antibody response to aggregated Aß using novel Alzheimer-vaccines. PLoS ONE 10, e0115237 (2015)
Michel M, Schwarten M, Decker C, Nagel-Steger L, Willbold D, Weiergräber OH The mammalian autophagy initiator complex contains 2 HORMA domain proteins Autophagy 11, 2300-2308 (2015) http://dx.doi.org/10.1080/15548627.2015.1076605
Pattky M, Nicolardi S, Santiago-Schübel B, Sydes D, van der Burgt YE, Klein AN, Jiang N, Mohrlüder J, Hänel K, Kutzsche J, Funke SA, Willbold D, Willbold S, Huhn C Structure characterization of unexpected covalent O-sulfonation and ion-pairing on an extremely hydrophilic peptide with CE-MS and FT-ICR-MS. Anal. Bioanal. Chem. 407, 6637-6655 (2015)
Shaykhalishahi H, Gauhar A, Wördehoff MM, Grüning CS, Klein A, Bannach O, Stoldt M, Willbold D, Härd T, Hoyer W Contact between the beta1 and beta2 segments of alpha-synuclein that inhibits amyloid formation Angew. Chem. Int. Ed. 54, 8837-8840 (2015) http://dx.doi.org/10.1002/anie.201503018
Shaykhalishahi H, Mirecka EA, Gauhar A, Grüning CSR, Willbold D, Härd T, Stoldt M, Hoyer W A beta-hairpin-binding protein for three different disease-related amyloidogenic proteins ChemBioChem 16, 411-414 (2015) http://dx.doi.org/10.1002/cbic.201402552
Solyom Z, Ma P, Schwarten M, Bosco M, Polidori A, Durand G, Willbold D, Brutscher B The Disordered Region of the HCV Protein NS5A: Conformational Dynamics, SH3 Binding, and Phosphorylation. Biophys. J. 109, 1483-1496 (2015)
Wolff M, Unuchek D, Zhang B, Gordeliy V, Willbold D, Nagel-Steger L Amyloid β oligomeric species present in the lag phase of amyloid formation. PLoS ONE 10, e0127865 (2015) http://dx.doi.org/10.1371/journal.pone.0127865
Aladag A, Hoffmann S, Stoldt M, Bösing C, Willbold D, Schwarten M Hepatitis C virus NS5A is able to competitively displace c-Myc from the Bin1 SH3 domain in vitro J. Pept. Science 20, 334-340 (2014)
Borshchevskiy V, Round E, Erofeev I, Weik M, Ishchenko A, Gushchin I, Mishin A, Willbold D, Büldt G, Gordeliy V Ultra-low dose X-ray radiation induces structural alterations in proteins Acta Cryst. D 70, 2675-2685 (2014)
Frenzel D, Glück JM, Brener O, Oesterhelt F, Nagel-Steger L, Willbold D Immobilization of homogeneous monomeric, oligomeric and fibrillar Aβ species for reliable SPR measurements PLoS ONE 9, e89490 (2014)
Frenzel D, Willbold D Kinetic titration series with biolayer interferometry PLoS ONE 9, e106882 (2014)
Grüning CS, Mirecka EA, Klein AN, Mandelkow E, Willbold D, Marino SF, Stoldt M, Hoyer W Alternative conformations of the tau repeat domain in complex with an engineered binding protein J. Biol. Chem. 289, 23209-23218 (2014) http://dx.doi.org/10.1074/jbc.M114.560920
Hänel K, Möckel L, Brummel M, Peiris K, Hartmann R, Dingley AJ, Willbold D, Loidl-Stahlhofen A Expression and purification of soluble HIV-2 viral protein R (Vpr) using a sandwich-fusion protein strategy Prot. Expr. Purific. 95, 156-161 (2014) http://www.sciencedirect.com/science/article/pii/S1046592813002763
Hung Y-F, Valdau O, Schünke S, Stern O, Koenig BW, Willbold D, Hoffmann S Recombinant production of the amino terminal cytoplasmic region of dengue virus non-structural protein 4A for structural studies PLoS ONE 9, e86482 (2014) Link
Ma P, Haller JD, Zajakala J, Macek P, Sivertsen AC, Willbold D, Boisbouvier J, Schanda P Probing transient conformational States of proteins by solid-state r1ρ relaxation-dispersion NMR spectroscopy. Angew. Chem. Int. Ed. Engl. 53, 4312–4317 (2014)
Mirecka EA, Gremer L, Schiefer S, Oesterhelt F, Stoldt M, Willbold D, Hoyer W Engineered aggregation inhibitor fusion for production of highly amyloidogenic human islet amyloid polypeptide J. Biotechnol. 191, 221-227 (2014) http://dx.doi.org/10.1016/j.jbiotec.2014.06.006
Müller H, Brener O, Andreoletti O, Piechatzek T, Willbold D, Legname G, Heise H Progress toward structural understanding of infectious sheep PrP-amyloid Prion 8, 344-358 (2014)
Olubiyi OO, Frenzel D, Bartnik D, Glück JM, Brener O, Nagel-Steger L, Funke SA, Willbold D, Strodel B Amyloid aggregation inhibitory mechanism of arginine-rich D-peptides Curr. Med. Chem. 21, 1448-1457 (2014)
Polovinkin V, Balandin T, Volkov O, Round E, Borshchevskiy V, Utrobin P, von Stetten D, Royant A, Willbold D, Arzumanyan G, Chupin V, Popot JL, Gordeliy V Nanoparticle Surface-Enhanced Raman Scattering of Bacteriorhodopsin Stabilized by Amphipol A8-35 J. Membr. Biol. 247, 997-980 (2014)
Polovinkin V, Gushchin I, Sintsov M, Round E, Balandin T, Chervakov P, Schevchenko V, Utrobin P, Popov A, Borshchevskiy V, Mishin A, Kuklin A, Willbold D, Chupin V, Popot JL, Gordeliy V High-Resolution Structure of a Membrane Protein Transferred from Amphipol to a Lipidic Mesophase J. Membr. Biol. 247, 997-1004 (2014)
Widera M, Klein NA, Cinar Y, Funke SA, Willbold D, Schaal H The D-amino acid peptide D3 reduces amyloid fibril boosted HIV-1 infectivity AIDS Res. Therapy 11, 1 (2014)
Arslan Z, Wurm R, Brener O, Ellinger P, Nagel-Steger L, Oesterhelt F, Schmitt L, Willbold D, Wagner R, Gohlke H, Smits S, Pul U Double-strand DNA end-binding and sliding of the toroidal CRISPR-associated protein Csn2. Nucl Acids Res 41, 6347-6359 (2013) open access
Bannach O, Reinartz E, Henke F, Dreßen F, Oelschlegel A, Kaatz M, Groschup M H, Willbold D, Riesner D and Birkmann E Analysis of prion protein aggregates in blood and brain from pre-clinical and clinical BSE cases Vet Microbiol 166, 102-108 (2013) http://dx.doi.org/10.1016/j.vetmic.2013.05.021
Chien T-H, Chiang Y-L, Chen C-P, Henklein P, Hänel K, Hwang I-S, Willbold D, Fischer WB Assembling an ion channel: ORF 3a from SARS-CoV Biopolymers 99, 628-635 (2013) http://dx.doi.org/10.1002/bip.22230
Do H, Wittlich M, Glück JM, Möckel L, Willbold D, Koenig BW, Heise H Full-length Vpu and human CD4(372-433) in phospholipid bilayers as seen by magic angle spinning NMR. Biol. Chem. 394, 1453-1463 (2013)
Dornieden S, Müller-Schiffmann A, Sticht H, Cinar Y, Wördehoff M, Korth C, Funke SA, Willbold D Characterization of a single-chain variable fragment recognizing a linear epitope of Aß: A biotechnical tool for studies on Alzheimer's disease? PLoS ONE 8, e59820 (2013)
Grüning CS, Klinker S, Wolff M, Schneider M, Toksöz K, Klein AN, Nagel-Steger L, Willbold D, Hoyer W The off-rate of monomers dissociating from amyloid-beta protofibrils J. Biol. Chem. 288, 37104-37111 (2013) http://dx.doi.org/10.1074/jbc.M113.513432
Kruse C, Willbold D, Schmitt L Highlight: NRW Research School BioStruct - Biological Structures in Molecular Medicine and Biotechnology. Biol. Chem. 394, 1353-1355 (2013)
Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S, Mohrlüder J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergräber OH, Willbold D Interaction of Bcl-2 with the autophagy-related GABAA receptor-associated protein (GABARAP): Biophysical characterization and functional implications J Biol Chem 288, 37204-37215 (2013)
Pavlidou M, Hänel K, Möckel L, Willbold D Nanodiscs allow phage display selection for ligands to non-linear epitopes on membrane proteins PLoS ONE 9, e72272 (2013)
Rani R, Jentzsch K, Lecher L, Hartmann R, Willbold D, Jaeger K-E, Krauss U Conservation of dark recovery kinetic parameters and structural features in the Pseudomonadaceae “short” LOV protein family – implications for the design of LOV-based optogenetic tools. Biochemistry 52, 4460-4473 (2013)
Schwarten M, Solyom Z, Feuerstein S, Aladag A, Hoffmann S, Willbold D, Brutscher B Interaction of non-structural protein 5A of hepatitis C virus with SH3 domains using non-canonical binding sites Biochemistry 52, 6160-6168 (2013)
Solyom Z, Schwarten M, Geist L, Konrat R, Willbold D, Brutscher B BEST-TROSY experiments for time-efficient sequential resonance assignment of large disordered proteins J. Biomol. NMR 55, 311-221 (2013)
Stern O, Hung Y-F, Valdau O, Yaffe Y, Harris E, Hoffmann S, Willbold D, Sklan E An N-terminal amphipathic helix in the Dengue virus nonstructural protein 4A mediates oligomerization and is essential for replication. J. Virol. 87, 4080-4085 (2013)
van Groen T, Kadish I, Funke SA, Bartnik D, Willbold D Treatment with D3 removes amyloid deposits, reduces inflammation, and improves cognition in old AβPP/PS1 double transgenic mice. J Alzheimer's Dis. 34, 609-620 (2013) open access
Wang-Dietrich L, Funke SA, Kühbach K, Wang K, Besmehn A, Willbold S, Cinar Y, Bannach O, Birkmann E, Willbold D The Aβ oligomer count in CSF is a biomarker for Alzheimer’s disease. J. Alzheimers Dis. 34, 985-994 (2013)
Weiergräber OH, Mohrlüder J, Willbold D Atg8 family proteins –- autophagy and beyond in: Autophagy - A double-edged sword - Cell survival or death? ISBN 978-953-51-1062-0, Editor: Yannick Bailly (2013) open access
Bannach O, Birkmann E, Reinartz E, Jaeger K-E, Langeveld JP, Rohwer RG, Gregory L, Terry LA, Willbold D, Riesner D Detection of Prion Protein Particles in Blood Plasma of Scrapie Infected Sheep PLoS ONE 7, e36620 (2012) http://dx.doi.org/10.1371/journal.pone.0036620 full text open access
Circolone F, Granzin J, Jentzsch K, Drepper T, Jaeger K-E, Willbold D, Krauss U, Batra-Safferling R Structural basis for the slow dark recovery of a full-length LOV protein from Pseudomonas putida. J. Mol. Biol. 417, 362–374 (2012)
Feuerstein S, Plevin MJ, Willbold D, Brutscher B iHADAMAC: a complementary tool for sequential resonance assignment of globular and highly disordered protein. J. Magn. Res. 214, 329-334 (2012)
Feuerstein S, Solyom Z, Aladag A, Favier A, Schwarten M, Hoffmann S, Willbold D, Brutscher B Transient structure and SH3 interaction sites in an intrinsically disordered fragment of the hepatitis C virus protein NS5A. J. Mol. Biol. 420, 310-323 (2012)
Funke SA, Bartnik D, Glück JM, Pirkowska K, Wiesehan K, Weber U, Gulyas B, Halldin C, Pfeifer A, Spenger C, Muhs A, Willbold D Development of a small D-enantiomeric Alzheimer's amyloid-beta binding peptide ligand for future in vivo imaging applications. PLoS ONE 7, e41457 (2012)
Funke SA, Liu H, Sehl T, Bartnik D, Brener O, Nagel-Steger L, Wiesehan K, Willbold D Identification and characterization of an Aβ oligomer precipitating peptide that may be useful to explore gene therapeutic approaches to Alzheimer’s disease. Rejuv. Res. 15, 144-147 (2012)
Funke SA, Willbold D Peptides for Therapy and Diagnosis of Alzheimer’s Disease. Curr. Pharm. Design 18, 755-767 (2012)
Gulyás B, Spenger C, Beliczai Z, Gulya K, Kása P, Jahan M, Jia Z, Weber U, Pfeifer A, Muhs A, Willbold D, Halldin C Distribution and binding of (18)F-labeled and (125)I-labeled analogues of ACI-80, a prospective molecular imaging biomarker of disease: A whole hemisphere post mortem autoradiography study in human brains obtained from Alzheimer's disease patients. Neurochem. Intern. 60, 153-162 (2012)
Holze R, Jacob T, Schünke S, Neudecker P, Willbold D Physical Chemistry 2011 Nachr. Chemie 60, 313-322 (2012)
Hübinger S, Bannach O, Funke SA, Willbold D, Birkmann E Detection of alpha-synuclein aggregates by fluorescence microscopy Rejuvenation Res. 15, 213-216 (2012)
Jahan M, Nag S, Krasikova R, Weber U, Muhs A, Pfeifer A, Spenger C, Willbold D, Gulyás B, Halldin C Fluorine-18 labeling of three novel d-peptides by conjugation with N-succinimidyl-4-[(18)F]fluorobenzoate and preliminary examination by postmortem whole-hemisphere human brain autoradiography. Nucl. Med. Biol. 392, 315-323 (2012)
Kammula E, Moetter J, Gorgels A, Jonas E, Hoffmann S, Willbold D Brain transcriptome-wide screen for HIV-1 Nef protein interaction partners reveals various membrane-associated proteins. PLoS ONE 7, e51578 (2012) http://dx.doi.org/10.1371/journal.pone.0051578 full text open access
Klionsky DJ, et al, Willbold D, et al (many co-authors) Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 8, 445-544 (2012)
Koenig BW, Schünke S, Stoldt M, Willbold D NMR methods for the determination of protein-ligand interactions. Protein-Ligand Interactions" (Editor: H. Gohlke) (series "Methods and Principles in Medicinal Chemistry"), Wiley-VCH; ISBN: 3-527-32966-8, (2012)
Kroth H, Ansaloni A, Varisco Y, Jan A, Sreenivasachary N, Rezaei-Ghaleh N, Giriens V, Lohmann S, Pilar López-Deber M, Adolfsson O, Pihlgren M, Paganetti P, Froestl W, Nagel-Steger L, Willbold D, Schrader T, Zweckstetter M, Pfeifer A, Lashuel HA, Muhs A Discovery and structure activity relationship of small molecule inhibitors of toxic β-amyloid-42 fibril formation. J. Biol. Chem. 287, 34786-34800 (2012)
Lecher J, Schwarz CKW, Stoldt M, Smits SHJ, Willbold D, Schmitt L RTX toxin transporters tether its substrate prior to secretion via the unique function of its N- terminal domain. Structure 20, 1778-1787 (2012)
Mazargui H, Lévêque C, Bartnik D, Fantini J, Gouget T, Melone MA, Funke SA, Willbold D, Perrone L A synthetic amino acid substitution of Tyr10 in Aβ peptide sequence yields a dominant negative variant in amyloidogenesis. Aging Cell 11, 530-541 (2012)
Singh S, Möckel L, Thiagarajan-Rosenkranz P, Wittlich M, Willbold D, Koenig B Mapping the interaction between the cytoplasmic domains of HIV-1 VpU and human CD4 using NMR spectroscopy. FEBS J. 279, 3705-3714 (2012)
Sun N, Funke SA, Willbold D A survey of peptides with effective therapeutic potential in Alzheimer’s disease rodent models or in human clinical studies. Mini. Rev. Med. Chem. 12, 388-398 (2012) full text open access
Sun N, Funke SA, Willbold D Mirror image phage display - Generating stable therapeutically and diagnostically active peptides with biotechnological means. J. Biotech. 161, 121-125 (2012) http://dx.doi.org/10.1016/j.jbiotec.2012.05.019 full text
Sun N, Hartmann R, Lecher J, Stoldt M, Funke SA, Gremer L, Ludwig H-H, Demuth H-U, Kleinschmidt M, Willbold D Structural analysis of the pyroglutamate modified isoform of the Alzheimer's disease related beta-amyloid using NMR spectroscopy. J. Pept. Science 18, 691-695 (2012)
van Groen T, Kadish I, Funke A, Bartnik D, Willbold D Treatment with Aβ42 Binding d-Amino Acid Peptides Reduce Amyloid Deposition and Inflammation in APP/PS1 Double Transgenic Mice. Adv. Protein. Chem. Struct. Biol. 88, 133-152 (2012)
Wang W, Pacheco V, Krause H-J, Zhang Y, Dong H, Hartmann R, Willbold D, Offenhaeusser A, Gu Z Size and compositional effects on contrast efficiency of functionalized superparamagnetic nanoparticles at ultralow and ultrahigh magnetic fields. J. Phys. Chem. C 116, 17880-17884 (2012) http://dx.doi.org/10.1021/jp302758h
Feuerstein S, Solyom Z, Aladag A, Hoffmann S, Willbold D, Brutscher B 1H, 13C, and 15N resonance assignment of a 179 residue fragment of hepatitis C virus nonstructural protein 5A Biomol. NMR Assign. 53, 241-243 (2011)
Funke SA, Willbold D Quantitation of Amyloid-β oligomers in human body fluids for Alzheimer’s disease early diagnosis or therapy monitoring. Alzheimer's diagnosis. Charles E. Ronson (Ed.), Hauppage, NY, USA (2011) Nova Science Publishers, ISBN 978-1-61209-846-3
Glück JM, Koenig BW, Willbold D Nanodiscs allow the use of integral membrane proteins as analytes in surface plasmon resonance studies. Anal. Biochem. 408, 46-52 (2011)
Hickman DT, López-Deber MP, Ndao DM, Silva AB, Nand D, Pihlgren M, Giriens V, Madani R, St-Pierre A, Karastan H, Nagel-Steger L, Willbold D, Riesner D, Nicolau C, Baldus M, Pfeifer A, Muhs A Sequence-independent control of peptide conformation in liposomal vaccines for targeting protein misfolding diseases. J. Biol.Chem. 286, 13966-13976 (2011)
Lecher J, Stoldt M, Schwarz CKW, Smits SHJ, Schmitt L, Willbold D ¹H, ¹⁵N and ¹³C resonance assignment of the N-terminal C39 peptidase-like domain of the ABC transporter Haemolysin B (HlyB). Biomol. NMR Assign. 53, 199-201 (2011)
Pfannkuche A, Büther K, Karthe J, Poenisch M, Bartenschlager R, Trilling M, Hengel H, Willbold D, Häussinger D, Bode JG c-Src is required for complex formation between the hepatitis C virus encoded proteins NS5A and NS5B – a prerequisite for replication. Hepatology 53, 1127-1136 (2011)
Schünke S, Stoldt M, Lecher J, Kaupp UB, Willbold D Structural insights into conformational changes of a cyclic nucleotide-binding domain in solution from Mesorhizobium loti K1 channel. Proc. Natl. Acad. Sci. USA 108, 6121-6126 (2011)
Schwarz CKW, Tschapek B, Jumpertz T, Jenewein S, Lecher J, Willbold D, Panjikar S, Halland B, Smits SHJ, Schmitt L Crystallisation and preliminary X-ray crystallographic studies of an oligomeric species of a refolded C39 peptidase-like domain of the Escherichia coli ABC transporter Haemolysin B Acta Cryst. F67, 630-633 (2011)
Stöhr J, Elfrink K, Weinmann N, Wille H, Willbold D, Birkmann E and Riesner D In vitro conversion of the posttranslationally modified prion protein. Biol. Chem. 392, 415-421 (2011)
van Groen T, Kadish I, Funke A and Willbold D Staining of Amyloid Beta (Abeta) Using (Immuno) Histochemical Techniques and Abeta42 Specific Peptides In: Neuroimaging for Clinicians - Combining Research and Practice. In Tech. Editor: Julio F. P. Peres; ISBN 978-953-307-450-4, Chapter 4 (2011) free download
Wang W, Dong H, Pacheco V, Willbold D, Zhang Y, Offenhausser A, Hartmann R, Weirich T, Ma P, Krause H, Gu Z Relaxation behavior study of ultra-small superparamagnetic iron oxide nanoparticles at ultra-low and ultra-high magnetic fields. J. Phys. Chem.B 115, 14789-14793 (2011)
Bartnik D, Funke SA, Andrei-Selmer L-C, Bacher M, Dodel R, Willbold D Differently selected D-enantiomeric peptides act on different Aß species. Rejuvenation Res. 13, 202-205 (2010)
Batra-Safferling R, Granzin J, Moedder S, Hoffmann S, Willbold D Structural studies of PI3K SH3 domain in complex with a peptide ligand: Role of anchor residue in ligand binding. Biol. Chem. 391, 33-42 (2010)
Funke SA, van Groen T, Kadish I, Bartnik D, Nagel-Steger L, Brener O, Sehl T, Batra-Safferling R, Moriscot C, Schoehn G, Horn AHC, Müller-Schiffmann A, Korth C, Sticht H, Willbold D Oral Treatment with the D-Enantiomeric Peptide D3 Improves Pathology and Behavior of Alzheimer’s disease Transgenic Mice ACS Chem. Neurosci. 1, 639-648 (2010) http://dx.doi.org/10.1021/cn100057j Highlighted in: Chemical & Engineering News, 88(33), August 16, 2010
Funke SA, Wang L, Birkmann E, Willbold D Single particle detection system for Aß: Adaptation of surface-FIDA to laser scanning microscopy. Rejuvenation Res. 13, 206-209 (2010)
Glück JM, Hoffmann S, Koenig BW, Willbold D Single Vector System for Efficient N-myristoylation of Recombinant Proteins in E. coli PLoS ONE 5, e10081 (2010) full text open access The plasmids in this publication can be received via https://www.addgene.org/Dieter_Willbold/
Hoffmann S, Funke SA, Wiesehan K, Moedder S, Glück JM, Feuerstein S, Gerdts M, Moetter J, Willbold D Competitively selected protein ligands pay theirs increase in specificity by a decrease in affinity. Mol. BioSyst 6, 116-123 (2010)
Liu HM, Funke SA, Willbold D Transport of Alzheimer's disease amyloid-ß peptide binding D-amino acid peptides across a blood-brain barrier in vitro model. Rejuvenation Res. 13, 210-213 (2010)
Ma P, Mohrlüder J, Schwarten M, Stoldt M, Singh SK, Hartmann R, Pacheco V, Willbold D Preparation of a functional GABARAP-lipid conjugate in nanodiscs and its investigation by solution NMR spectroscopy Chembiochem 11, 1967-1970 (2010)
Müller-Schiffmann A, März-Berberich J, Andrjevna A, Rönicke R, Bartnik D, Brener O, Kutzsche J, Horn AHC, Hellmert M, Polkowska J, Gottmann K, Reymann K, Funke SA, Nagel-Steger L, Moriscot C, Schoehn G, Sticht H, Willbold D, Schrader T, Korth C Combining independent drug classes into superior, synergistically acting hybrid molecules. Angew. Chem. Int. Ed. Engl. 49, 8743-8746 (2010) http://dx.doi.org/10.1002/anie.201004437
Nagel-Steger L, Demeler B, Meyer-Zaika W, Hochdörffer K, Schrader T, Willbold D Modulation of aggregate size- and shape-distributions of the amyloid-beta peptide by a designed ß-sheet breaker. Eur. Biophys. J. 39, 415-422 (2010) http://dx.doi.org/10.1007/s00249-009-041
Pacheco V, Ma P, Thielmann Y, Hartmann R, Weiergräber OH, Mohrlüder J, Willbold D Assessment of GABARAP self-association by its diffusion properties J Biomol NMR 48, 49-58 (2010)
Panza G, Luers L, Stöhr J, Nagel-Steger L, Weiβ J, Riesner D, Willbold D, Birkmann E Molecular interactions between prions as seeds and recombinant prion proteins as substrates resemble the biological interspecies barrier in vitro. PLoS ONE 5, e14283 (2010) http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0014283
Schünke S, Lecher J, Stoldt M, Kaupp UB, Willbold D Resonance assignments of the nucleotide-free wildtype MloK1 cyclic nucleotide-binding domain. Biomol. NMR Assign. 4, 147-150 (2010)
Schwarten M, Stoldt M, Mohrlüder J, Willbold D Solution structure of Atg8 reveals conformational polymorphism of the N-terminal domain Biochem Biophys Res Commun 395, 426-431 (2010)
Wittlich M, Thiagarajan P, Koenig BW, Hartmann R, Willbold D NMR structure of the transmembrane and cytoplasmic domains of human CD4 in micelles. Biochim Biophys Acta 1798, 122-127 (2010)
Feuerstein SE, Pulvermüller A, Hartmann R, Granzin J, Stoldt M, Henklein P, Ernst OP, Heck M, Willbold D, Koenig BW Helix formation in Arrestin accompanies recognition of photoactivated Rhodopsin. Biochemsitry 48, 10733-42 (2009)
Funke SA, Birkmann E, Willbold D Detection of Amyloid-β aggregates in body fluids: A suitable method for early diagnosis of Alzheimer's diesease? Curr. Alzheimer Res. 6, 285-289 (2009)
Funke SA, Willbold D Mirror image phage display - a method to generate D-peptide ligands for use in diagnostic or therapeutical applications? Mol. BioSyst. 5, 783-786 (2009)
Glück JM, Wittlich M, Feuerstein S, Hoffmann S, Willbold D, Koenig BW Integral membrane proteins in nanodiscs can be studied by solution NMR spectroscopy. J. Am. Chem. Soc. 131, 12060-61 (2009)
Görtz P, Opatz J, Siebler M, Funke SA, Willbold D, Lange-Asschenfeldt C Transient reduction of spontaneous neuronal network activity by sublethal amyloid beta (1-42) peptide concentrations. J. Neural Transmission 116, 351-355 (2009)
Leliveld SR, Hendriks P, Michel M, Sajnani G, Bader V, Trossbach S, Prikulis I, Hartmann R, Jonas E, Willbold D, Requena JR, Korth C Oligomer assembly of the C-terminal DISC1 domain (640-854) is controlled by self-association motifs and disease-associated polymorphism S704C. Biochemistry 48, 7746-7755 (2009)
Mangels C, Frank AO, Ziegler J, Klingenstein R, Schweimer K, Willbold D, Korth C, Rösch P, Schwarzinger S Binding of TCA to the prion protein: mechanism, implication for therapy, and application as probe for complex formation of bio-macromolecules. J. Biomol. Struct. Dyn. 27, 163-170 (2009)
Mohrlüder J, Schwarten M, Willbold D Structure and potential function of gamma-aminobutyrate type A receptor-associated protein FEBS J 276, 4989-5005 (2009)
Schlenzig D, Manhart S, Cinar Y, Willbold D, Funke SA, Kleinschmidt M, Hause G, Schilling S, Demuth H-U Pyroglutamate formation influences solubility and amyloidenicity of amyloid peptides. A driving force in different neurodegenerative disorders? Biochemistry 48, 7072-7078 (2009)
Schünke S, Stoldt M, Novak K, Kaupp UB, Willbold D Solution structure of the M.loti K1 channel cyclic nucleotide binding domain in complex with cAMP. EMBO Rep. 10, 729-735 (2009)
Schwarten M, Mohrlüder J, Ma P, Stoldt M, Thielmann Y, Stangler T, Hersch N, Hoffmann B, Merkel R, Willbold D Nix directly binds to GABARAP: a possible crosstalk between apoptosis and autophagy Autophagy 5, 690-698 (2009)
Schwarten M, Stoldt M, Mohrlüder J, Willbold D Sequence-specific 1H, 13C, and 15N resonance assignment of the autophagy-related protein Atg8 Biomol NMR Assign 3, 137-139 (2009)
Thielmann Y, Weiergräber OH, Ma P, Schwarten M, Mohrlüder J, Willbold D Comparative modeling of human NSF reveals a possible binding mode of GABARAP and GATE-16 Proteins 77, 637-646 (2009)
Thielmann Y, Weiergräber OH, Mohrlüder J, Willbold D Structural characterization of GABARAP-ligand interactions Mol Biosyst 5, 575-579 (2009)
Thielmann Y, Weiergräber OH, Mohrlüder J, Willbold D Structural framework of the GABARAP-calreticulin interface - implications for substrate binding to endoplasmic reticulum chaperones FEBS J 276, 1140-1152 (2009)
van Groen T, Kadish I, Wiesehan K, Funke, SA, Willbold D In vitro and in vivo staining characteristics of small, fluorescent, Aß42 binding D-enantiomeric peptides in transgenic AD mouse models. ChemMedChem 4, 276-282 (2009)
Wittlich M, Koenig BW, Stoldt M, Schmidt H, Willbold D NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles. FEBS J. 276, 6560-75 (2009)
Birkmann E, Funke SA, Riesner D, Willbold D Single particle Detection - a diagnostic tool for particle associated diseases like Alzheimer’s disease and Creutzfeldt-Jakob disease, INSTICC, Proceedings of Biosignals 2, 431-436 (2008)
Birkmann E, Henke F, Funke SA, Bannach O, Riesner D, Willbold D A highly sensitive diagnsotic assay for aggregate-related diseases e.g. prion disease and Alzheimer's disease. Rejuvenation Res. 11, 359-363 (2008)
Elfrink K, Ollesch J, Stoehr J, Willbold D, Riesner D, Gerwert K Structural changes of PrPc upon membrane anchoring. Proc. Natl. Acad. Sci. 105, 10815-10819 (2008)
Funke SA, Birkmann E, Henke F, Görtz P, Lange-Asschenfeldt C, Riesner D, Willbold D An ultra sensitive assay for early diagnosis of Alzheimer's disease. Rejuvenation Res. 11, 315-318 (2008)
Hartmann R, Koenig BW, Stangler T, Willbold D Structural Proteomics: Exploring Protein-Ligand Interactions by Solution NMR. in 'Proteomics Sample Preparation', J. v. Hagen, Wiley-VCH , 273-281 (2008)
Mueller CB, Loman A, Pacheco V, Koberling F, Willbold D, Richtering W, Enderlein J Precise measurement of diffusion by multi-color dual-focus fluorescence correlation spectroscopy. Europhys. Lett. 83, 46001 (2008)
Nagel-Steger L, Demeler B, Hochdörfer K, Schrader T, Willbold D Modulation of aggregate size and shape distributions of amyloid-ß peptide solutions by a designed ß-sheet breaker. NIC Workshop 2008 (From Computational Biophysics to Systems Biology; CBSB08) Vol. 40, (2008) http://www.fz-juelich.de/nic-series/volume40/nagel_steger.pdf
Panza G, Stöhr J, Birkmann E, Riesner D, Willbold D, Baba O, Terashima T, Dumpitak C Aggregation and amyloid fibril formation of the prion protein is accelerated in presence of glycogen. Rejuvenation Res. 11, 365-369 (2008)
Panza G, Stöhr J, Dumpitak C, Papathanassiou D, Weinmann N, Weiß J, Riesner D, Willbold D, Birkmann E Spontaneous and BSE-prion-seeded amyloid formation of full length recombinant bovine Prion Protein. Biochem. Biophys. Research Comm. 373, 493.497 (2008)
Thielmann Y, Mohrlüder J, Koenig BW, Stangler T, Hartmann R, Becker K, Höltje HD, Willbold D An indole binding site is a major determinant of the ligand specificity of the GABA type A receptor-associated protein GABARAP Chembiochem 9, 1767-1775 (2008)
Weiergräber OH, Stangler T, Thielmann Y, Mohrlüder J, Wiesehan K, Willbold D Ligand binding mode of GABAA receptor-associated protein J Mol Biol 381, 1320-1331 (2008)
Wiesehan K, Stöhr J, Nagel-Steger K, van Groen T, Riesner D and Willbold D Inhibition of cytotoxicity and fibril formation by a D-amino acid peptide that specifically binds to Alzheimer's disease amyloid peptide Prot. Eng. Des. Sel. 21, 241-246 (2008) http://dx.doi.org/10.1093/protein/gzm054
Wittlich M, Koenig BW, Willbold D Structural consequences of phosphorylation of two serine residues in the cytoplasmic domain of HIV-1 VpU. J. Pept. Sci. 14, 804-10 (2008)
Birkmann E, Henke F, Weinmann N, Dumpitak C, Groshup M, Funke SA, Willbold D, Riesner D, Counting of single prion particles bound to a capture-antibody surface (surface-FIDA), Vet. Microbiol. 123, 294-304 (2007)
Funke SA, Birkmann E, Henke F, Görtz P, Lange-Asschenfeldt C, Riesner D, Willbold D, Single particle detection of amyloid-ß aggregates associated with Alzheimer's disease. Biochem. Biophys. Res. Comm. 364, 902-907 (2007)
Hänel K, Willbold D SARS-CoV accessory protein 7a directly interacts with human LFA-1. Biol. Chem. 388, 1325-1332 (2007)
Hoffmann S, Jonas E, Konig S, Preusser-Kunze A, Willbold D Nef protein of human immunodeficiency virus type 1 binds its own myristoylated N-terminus. Biol. Chem. 366, 181-183 (2007)
Mohrlüder J, Hoffmann Y, Stangler T, Hänel K, Willbold D Identification of clathrin heavy chain as a direct interaction partner for the gamma-aminobutyric acid type A receptor associated protein Biochemistry 46, 14537-14543 (2007)
Mohrlüder J, Stangler T, Wiesehan K, Hoffmann Y, Mataruga A, Willbold D Identification of calreticulin as a ligand of GABARAP by phage display screening of a peptide library FEBS J 274, 5543-5555 (2007)
Nagel-Steger L, Demeler B, Willbold D Aggregate size and shape distributions in amyloid-ß peptide solutions. Aggregate Size and Shape Distributions in Amyloid-beta Peptide Solutions. NIC Workshop (From Computational Biophysics to Systems Biology; CBSB07) Vol. 36, (2007) http://www.fz-juelich.de/nic-series/volume36/nagel-steger.pdf
Schmidt H, Hoffmann S, Tran T, Stoldt M, Stangler T, Wiesehan K, Willbold D Solution structure of a Hck SH3 domain ligand complex reveals novel interaction modes. J. Mol. Biol. 365, 1517-1532 (2007)
Schünke S, Novak K, Stoldt M, Kaupp UB, Willbold D Resonance assignment of the cyclic nucleotide binding domain from a cyclic nucleotide-gated K+ channel in complex with cAMP. Biomol. NMR Assign. 1, 179-181 (2007)
Stangler T, Hartmann R, Willbold D, Koenig BW Modern high resolution NMR for the study of structure, dynamics and interactions of biological macromolecules. In: 'Progress in Physical Chemistry', Vol. 1 / Different Aspects of Intermolecular Interaction, Oldenbourg, München , 111-157 (2007)
Stangler T, Tran T, Hoffmann S, Schmidt H, Jonas E, Willbold D Competitive displacement of full-length HIV-1 Nef from Hck SH3 domain by a high affinity artificial peptide. Biol. Chem. 388, 611-615 (2007)
Wiesehan K, Funke SA, Fries M, Willbold D Purification of recombinantly expressed and cytotoxic human amyloid-beta peptide. J. Chrom. B 856, 229-233 (2007)
Wittlich M, Koenig BW, Hoffmann S, Willbold D Structural characterisation of the transmembrane and cytoplasmic domains of human CD4. Biochim Biophys Acta 1768, 2949-60 (2007)
Wittlich M, Wiesehan K, Koenig BW, Willbold D Expression, purification, and membrane reconstitution of a CD4 fragment comprising the transmembrane and cytoplasmic domains of the receptor. Protein Expr. Purif. 55, 198-207 (2007)
Batra-Safferling R, Abaraca-Heidemann K, Körschen HG, Tziatzios C, Stoldt M, Budyak I, Willbold D, Schwalbe H, Klein-Seetharaman J, Kaupp UB Glutamic acid-rich proteins of rod photoreceptors are natively unfolded. J Biol Chem 281, 1449-1460 (2006)
Hänel K, Stangler T, Stoldt M, Willbold D Solution structure of the X4 protein coded by the SARS related coronavirus reveals an immunoglobulin like fold and suggests a binding activity to integrin I domains. J. Biomed. Sci. 13, 281-293 (2006)
Lemaitre V, Wray V, Willbold D, Watts A, Fischer WB Full length Vpu from HIV-1: Combining molecular dynamics simulations with NMR spectroscopy. J. Biomol. Struct. Dyn. 23, 485-496 (2006)
Psurek A, Feuerstein S, Willbold D, Scriba GKE Effects of the secondary structure of amphiphilic a-helical peptides on separation selectivity in nonaqueous compared to aqueous capillary electrophoresis. Electrophoresis 27, 1868-1775 (2006)
Schwarzinger S, Willbold D, Ziegler J Structural Studies of Prion Proteins. in 'Prions and prion diseases', B. Hörnlimann, D. Riesner, H. Kretzschmer,eds. de Gruyter, Berlin , (2006)
Stangler T, Hartmann R, Willbold D, Koenig BW Modern high resolution NMR for the study of structure, dynamics and interactions of biological macromolecules. Z. Phys. Chem. 220, 567-613 (2006)
Briese L, Preusser A, Willbold D Mapping the binding site of full length HIV-1 Nef on human Lck SH3 by NMR spectroscopy. J. Biomed. Sci. 12, 451-456 (2005)
Harrer E, Bäuerle M, Ferstl B, Chaplin P, Petzold B, Mateo L, Handley A, Tzatzaris M, Vollmar J, Bergmann S, Rittmaier M, Eismann K, Müller S, Kalden JR, Spriewald B, Willbold D, Harrer T Therapeutic vaccination of HIV-1-infected patients on HAART with a recombinant HIV-1 nef-expressing MVA: safety, immunogenicity and influence on viral load during treatment interruption. Antiviral Therapy 10, 285-300 (2005)
Tran T, Hoffmann S, Wiesehan K, Jonas E, Luge C, Aladag A, Willbold D Insights into human Lck SH3 domain binding specificity: different binding modes of artificial and native ligands. Biochemistry 44, 15042-15052 (2005)
Willbold D Interaction of HIV-1 Nef with human CD4 and Lck. in 'Viral membrane proteins: structure, function and drug design', W. Fischer, ed. Kluwer Academic Publishers, New York , (2005)
Willbold D, Koenig BW NMR Analysis of Biopolymer Structure. In 'Physics meets Biology: From Soft Matter to Cell Biology', G. Gompper, UB. Kaupp, JKG Dhont, D Richter, RG Winkler,eds. , B3.1-B3.14 (2004)
Briese L and Willbold D Structure determination of human Lck unique and SH3 domains by nuclear magentic resonance spectroscopy. BMC Struct. Biol. 3, 3 (2003)
Preusser A, Jonas G and Willbold D Purification of recombinantly expressed human cluster determinant 4 cytoplasmatic domain. J. Chromatogr. B 786, 39-44 (2003)
Wiesehan K, Buder K, Linke RP, Patt S, Stoldt M, Unger E, Schmitt B, Bucci E and Willbold D Selection of D-amino-acid peptides that bind to Alzheimer's disease amyloid peptide Abeta(1-42) by mirror image phage display. ChemBioChem 4, 748-753 (2003)
Wiesehan K, Willbold D Mirror image phage display: Aiming at the mirror. ChemBioChem 4, 811-815 (2003)
Engler A, Stangler T, Willbold D Structure of human immunodeficiency virus type 1 Vpr (34-51) peptide in micelle containing aqueous solution. Eur. J. Biochem. 269, 3264-3269 (2002)
Preusser A, Briese L, Willbold D Presence of a helix in human CD4 cytoplasmic domain promotes binding to HIV-1 Nef protein. Biochem. Biochem. Biophys. Res. Comm. 292, 734-740 (2002)
Stangler T, Mayr LM, Willbold D Solution structure of the GABAA receptor associated protein GABARAP: Implications for biological function and its regulation. J. Biol. Chem. 277, 13363-13366 (2002)
Briese L, Hoffmann S, Friedrich U, Biesinger B, Willbold D Sequence-specific 1H, 13C and 15N resonance assignments of lymphocyte specific kinase unique and SH3 domains. J. Biomol. NMR 19, 193-194 (2001)
Engler A, Stangler T, Willbold D Solution structure of human immunodeficiency virus type 1 Vpr (13-33) peptide in micelles. Eur. J. Biochem. 268, 389-395 (2001)
Jonas G, Hoffmann S, Willbold D Binding of phage displayed HIV-1 Tat to TAR RNA in the presence of Cyclin T1. J. Biomed. Sci. 8, 340-346 (2001)
Preusser A, Briese L, Baur AS, Willbold D Direct in vitro binding of full-length HIV-1 Nef protein to CD4 cytoplasmic domain. J. Virol. 75, 3960-3964 (2001)
Willbold D & Wiesehan K Peptid zur Diagnose und Therapie der Alzheimer-Demenz National: DE 10117281A1, international: PCT/EP02/03862 , (2001)
Rosin-Arbesfeld R, Willbold D, Yaniv A, Gazit A The Tat protein of equine infectious anemia virus (EIAV) activates cellular gene expression by read-through transcription. Gene 219, 25-35 (1998)
Seewald MJ, Metzger AU, Willbold D, Rösch P, Sticht H Structural model of the HIV-1 Tat(46-58)-TAR complex. J. Biomol. Structure & Dynamics 16, 683-692 (1998)
Willbold D, Metzger AU, Sticht H, Gallert KC, Voit R, Dank N, Bayer P, Krauss G, Goody RS, Rösch P Equine infectious anemia virus transactivator is a homeodomain type protein. J. Mol. Biol. 277, 749-755 (1998)
Baur AS, Sass G, Laffert B, Willbold D, Mayer C, Peterlin BM The N-terminus of Nef from HIV-1 associates with a protein complex containing Lck and a serine kinase. Immunity 6, 283-291 (1997)
Hoffmann S, Willbold D A selection system to study protein-RNA interactions: Functional display of HIV-1 Tat protein on filamentous bacteriophage M13 Biochem. Biophys. Res. Comm. 235, 806-811 (1997)
Metzger AU, Bayer B, Willbold D, Hoffmann S, Frank RW, Goody RS, Rösch P The interaction of HIV-1 Tat (32-72) with its target RNA: A Fluorescence and Nuclear Magnetic Resonance Study Biochem. Biophys. Res. Comm. 241, 31-36 (1997)
Metzger AU, Bayer P, Willbold D, Hoffmann S, Frank RW, Goody RS, Rösch P The interaction of HIV-1 Tat (32-72) with its target RNA: A Fluorescence and Nuclear Magnetic Resonance Study. Biochem. Biophys. Res. Comm. 241, 31-36 (1997)
Metzger AU, Heckel M, Willbold D, Breitschopf K, RajBhandary UL, Rösch P, Gross HJ Structural studies on tRNA acceptor stem microhelices: exchange of the discriminator base A73 for G in human tRNALeu switches the acceptor specificity from leucine to serine by degreasing the stability of the terminal G1-C72 basepair. Nucl. Acids Res. 35, 4551-4556 (1997)
Willbold D, Hoffmann S, Rösch P Secondary structure and tertiary fold of the human immunodeficiency virus protein U (Vpu) cytoplasmic domain in solution Eur. J. Biochem. 245, 581-588 (1997)
Kirsch T, Boehm M, Schuckart O, Metzger AU, Willbold D, Frank RW, Rösch P Structural studies on the equine infectious anemia virus trans-activator protein. Eur. J. Biochem. 240, 75-84 (1996)
Metzger AU, Schindler T, Willbold D, Kraft M, Steegborn C, Volkmann A, Frank RW, Rösch P Structural rearrangements on HIV 1 Tat (32-72) TAR complex formation. FEBS Letters 384, 225-259 (1996)
Rösch P, Willbold D Is EIAV Tat protein a homeodomain? Science 272, 1672 (1996)
Willbold D, Rösch P Solution structure of the human CD4 (403-419) receptor peptide. J. Biomed. Sci. 3, 435-441 (1996)
Sticht H, Bayer P, Willbold D, Dames S, Hilbich C, Beyreuther K, Frank RW, Rösch P Structure of amyloid A4-(1-40)-peptide of Alzheimer’s desease. Eur. J. Biochem. 233, 293-298 (1995)
Mayr LM, Willbold D, Landt O, Schmid FX Role of the Cys2-Cys10 disulfide bond for the structure, stability, and folding kinetics of ribonuclease T1. Protein Science 3, 227-239 (1994)
Mayr LM, Willbold D, Rösch P, Schmid FX Generation of a non-prolyl cis peptide bond in ribonuclease T1. J. Mol. Biol. 240, 288-293 (1994)
Rosin-Arbesfeld R, Mashiah P, Willbold D, Rösch P, Tronick SR, Yaniv A, Gazit A Biological activity and intracellular location of the Tat protein of equine infectious anemia virus. Gene 150, 307-311 (1994)
Sticht H, Willbold D, Bayer P, Ejchart A, Herrmann F, Rosin-Arbesfeld R, Gazit A, Yaniv A, Frank R, Rösch P EIAV Tat is a Predominantly Helical Protein. Eur. J. Biochem. 218, 973-976 (1994)
Sticht H, Willbold D, Ejchart A, Rosin-Arbesfeld R, Rösch P Trifluoroethanol stabilizes a helix-turn-helix motif in equine infectious anemia virus Tat protein. Eur. J. Biochem. 255, 855-861 (1994)
Sticht H, Willbold D, Rösch P Molecular dynamics simulation of equine infectious anemia virus Tat protein in water and in 40 % trifluoroethanol. J. Biomol. Struct. Dyn. 12, 19.36 (1994)
Willbold D, Rosin-Arbesfeld R, Sticht H, Frank R, Rösch P Structure of the equine infectious anemia virus Tat protein. Science 264, 1584-1587 (1994)
Willbold D, Krüger U, Frank R, Rosin-Arbesfeld R, Gazit A, Yaniv A, Rösch P Sequence specific resonance assignments of the 1H-NMR spectra of a synthetic, biologically active EIAV-Tat protein. Biochemistry 32, 8439-8445 (1993)
