Agerschou ED, Schützmann MP, Reppert N, Wördehoff MM, Shaykhalishahi H, Buell AK, Hoyer W β-Turn exchanges in the α-synuclein segment 44-TKEG-47 reveal high sequence fidelity requirements of amyloid fibril elongation Biophys. Chem. 269, 106519 (2021) https://doi.org/10.1016/j.bpc.2020.106519
Agerschou ED, Flagmeier P, Saridaki T, Galvagnion C, Komnig D, Heid L, Prasad V, Shaykhalishahi H, Willbold D, Dobson CM, Voigt A, Falkenburger B, Hoyer W, Buell AK An engineered monomer binding-protein for α-synuclein efficiently inhibits the proliferation of amyloid fibrils eLife 8, e46112 (2019) https://doi.org/10.7554/eLife.46112
Falke M, Victor J, Wördehoff MM, Peduzzo A, Zhang T, Schröder GF, Buell AK, Hoyer W, Etzkorn M α-Synuclein-derived lipoparticles in the study of α-synuclein amyloid fibril formation Chem Phys Lipids 220, 57-65 (2019) https://doi.org/10.1016/j.chemphyslip.2019.02.009
Alexander K. Buell The nucleation of protein aggregates – from crystals to fibrils International Review of Cell and Molecular Biology , (2016) http://dx.doi.org/10.1016/bs.ircmb.2016.08.014
Anđela Šarić, Alexander K. Buell, Georg Meisl, Thomas C. T. Michaels, Sara Linse, Tuomas P. J. Knowles and Daan Frenkel Physical determinants for the self-replication of protein fibrils Nature Physics 12, 874-880 (2016) http://www.nature.com/nphys/journal/v12/n9/full/nphys3828.html
Céline Galvagnion, James Brown, Myriam M. Ouberai, Patrick Flagmeier, Michele Vendruscolo, Alexander K. Buell, Emma Sparr and Christopher M. Dobson The chemical properties of lipids strongly affect the rate of the nucleation step in the membrane-induced aggregation of α-synuclein PNAS 113(26), 7065-7070 (2016) http://www.pnas.org/content/113/26/7065
Dorothea Pinotsi, Claire H. Michel, Alexander K. Buell, Christopher M. Dobson, Clemens F. Kaminski, Gabriele S. Kaminski Schierle Nanoscopic insights into seeding mechanisms and toxicity of α-synuclein species in neurons PNAS 113(14), 3815-3819 (2016) http://www.pnas.org/content/113/14/3815
Giulio Costantini, Zoe Budrikis, Alessandro Taloni, Alexander K. Buell, Stefano Zapperi and Caterina A.M. La Porta Fluctuations in protein aggregation: Design of preclinical screening for early diagnosis of neurodegenerative disease Phys Rev Appl 6, 034012 (2016) http://journals.aps.org/prapplied/abstract/10.1103/PhysRevApplied.6.034012
James Brown, Alexander K. Buell, Thomas C.T. Michaels, Georg Meisl, Jackie Carozza, Patrick Flagmeier, Michele Vendruscolo, Tuomas P.J. Knowles, Christopher M. Dobson, Céline Galvagnion β-synuclein suppresses both the initiation and amplification steps of α-synuclein aggregation via competitive binding Scientific Reports 6, 36010 (2016) http://www.nature.com/articles/srep36010
Manuel Wolff, Judith J. Mittag, Therese W. Herling, Erwin De Genst, Christopher M. Dobson, Tuomas P.J. Knowles, Dieter Braun and Alexander K. Buell Quantitative thermophoretic study of disease-related protein aggregates Scientific Reports 6, 22829 (2016) http://www.nature.com/articles/srep22829
Patrick Flagmeier, Georg Meisl, Michele Vendruscolo, Tuomas PJ Knowles, Christopher M. Dobson, Alexander K. Buell and Céline Galvagnion Mutations associated with familial Parkinson’s disease alter the initiation and amplification steps of α-synuclein amyloid formation PNAS 113(37), 10328-10333 (2016) http://www.pnas.org/content/113/37/10328
Thomas O. Mason, Thomas C.T. Michaels, Aviad Levin, Ehud Gazit, Christopher M. Dobson, Alexander K. Buell and Tuomas P. J. Knowles Synthesis of non-equilibrium supra-molecular peptide polymers on a microfluidic platform JACS 138(30), 9589-9596 (2016) http://pubs.acs.org/doi/abs/10.1021/jacs.6b04136
Yingbo Zhang, Alexander K. Buell, Thomas Müller, Erwin De Genst, Justin Benesch, Christopher M. Dobson and Tuomas P. J. Knowles Protein aggregate-ligand binding assays based on microfluidic diffusional separation ChemBioChem 17(20), 1920-1924 (2016) http://onlinelibrary.wiley.com/doi/10.1002/cbic.201600384/abstract
Céline Huynen, Nicolas Willet, Alexander K. Buell, Anne-Sophie Duwez, Christine Jerôme and Mireille Dumoulin Influence of the protein context on the polyQ length dependent elongation rate of amyloid fibrils Biochim. Biophys. Acta – Proteins & Proteomics 1854(3), 239-248 (2015) http://www.sciencedirect.com/science/article/pii/S1570963914003185
Galvagnion C, Buell AK, Meisl G, Michaels TCT, Vendruscolo M, Knowles TPJ and Dobson CM Lipid vesicles trigger α-synuclein aggregation by stimulating primary nucleation. Nat. Chem. Biol. 11, 229-234 (2015) Link to full text
Shimanovich U, Efimov I, Mason TO, Flagmeier P, Buell AK, Gedanken A, Dobson CM, Weitz DA and Knowles TPJ Controlled structuring of protein-only amyloid nanofibril networks into 3D microgel particles ACS Nano 9(1), 43-51 (2015) Link to full text
Thomas C.T. Michaels, Pernille Yde, Julian C.W. Willis, Mogens H. Jensen, Daniel E. Otzen, Christopher M. Dobson, Alexander K. Buell and Tuomas P.J. Knowles The length distribution of frangible biofilaments J Chem Phys 143, 164901 (2015) http://aip.scitation.org/doi/abs/10.1063/1.4933230?journalCode=jcp
Alexander K. Buell, Céline Galvagnion, Ricardo Gaspar, Emma Sparr, Michele Vendruscolo, Tuomas P.J. Knowles, Sara Linse and Christopher M. Dobson Solution conditions determine the relative importance of nucleation and growth processes in α-synuclein aggregation PNAS 111(21), 7671-7676 (2014) http://www.pnas.org/content/111/21/7671
Alexander K. Buell, Christopher M. Dobson and Tuomas P.J. Knowles The physical chemistry of the amyloid phenomenon - thermodynamics and kinetics of filamentous protein aggregation Essays Biochem 56(1), 11-39 (2014) http://essays.biochemistry.org/content/56/11
Aviad Levin, Thomas Mason, Lihi Adler-Abramovich, Alexander K. Buell, Georg Meisl, Céline Galvagnion, Yaron Bram, Samuel A. Stratford, Christopher M. Dobson, Tuomas P.J. Knowles and Ehud Gazit Ostwald's rule of stages governs structural transitions and morphological control of a dipeptide supramolecular polymer Nature Communications 5, 5219 (2014) http://www.nature.com/articles/ncomms6219
Dorothea Pinotsi, Alexander K. Buell, Celine Galvagnion, Christopher M. Dobson, Gabriele S. Kaminski Schierle and Clemens F. Kaminski Direct observation of heterogeneous amyloid fibril growth kinetics via two-color super-resolution microscopy Nano Letters 14(1), 339-345 (2014) http://pubs.acs.org/doi/abs/10.1021/nl4041093
Nikolai Lorenzen, Søren Bang Nielsen, Alexander K. Buell, Jørn Døvling Kaspersen, Paolo Arosio, Brian Stougaard Vad, Gunna Christiansen, Zuzana Valnickova-Hansen, Maria Andreasen, Wojciech Paslawski, Jan J. Enghild, Christopher M. Dobson, Tuomas P. J. Knowles, Jan Skov Pedersen and Daniel Erik Otzen The role of stable α-synuclein oligomers in the molecular events underlying amyloid formation JACS 136, 3859-3868 (2014) http://pubs.acs.org/doi/abs/10.1021/ja411577t
Samuel I. A. Cohen, Luke Rajah, Chang-Ho Yoon, Alexander K. Buell, Duncan A. White, Ralf A. Sperling, Michele Vendruscolo, Eugene M. Terentjev, Christopher M. Dobson, David A. Weitz and Tuomas P. J. Knowles Spatial propagation of protein polymerisation Physical Review Letters 12, 098101 (2014) http://journals.aps.org/prl/abstract/10.1103/PhysRevLett.112.098101
Thomas C.T. Michaels, Alexander K. Buell, Eugene M. Terentjev and Tuomas P.J. Knowles Quantitative Analysis of Diffusive Reactions at the Solid–Liquid Interface in Finite Systems J Phys Chem Lett 5, 695-699 (2014) http://pubs.acs.org/doi/abs/10.1021/jz4024833
Thomas O. Mason, Dimitri Y. Chirgadze, Aviad Levin, Lihi Adler-Abramovich, Ehud Gazit, Tuomas P.J. Knowles and Alexander K. Buell Expanding the solvent chemical space for self-assembly of dipeptide nanostructures ACS Nano 8, 1243-1253 (2014) http://pubs.acs.org/doi/abs/10.1021/nn404237f
Alexander K. Buell, Peter Hung, Xavier Salvatella, Mark E. Welland, Christopher M. Dobson and Tuomas P. J. Knowles Electrostatic effects in filamentous protein aggregation Biophysical Journal 104, 1116-1126 (2013) http://www.cell.com/biophysj/abstract/S0006-3495(13)00136-7
Andrea Pica, Antonello Merlino, Alexander K. Buell, Tuomas P.J. Knowles, Elio Pizzod, Giuseppe D'Alessio, Filomena Sica and Lelio Mazzarella 3D domain swapping and supramolecular protein assembly: insights from the X-ray structure of a dimeric swapped variant of human pancreatic RNase Acta Cryst. D 69, 2116-2123 (2013) http://scripts.iucr.org/cgi-bin/paper?S0907444913020507
Dorothea Pinotsi, Alexander K. Buell, Christopher M. Dobson, Gabriele S. Kaminski Schierle and Clemens F. Kaminski A Label-Free, Quantitative Assay of Amyloid Fibril Growth Based on Intrinsic Fluorescence ChemBioChem 14, 846-850 (2013) http://onlinelibrary.wiley.com/doi/10.1002/cbic.201300103/abstract
Li-Qiong Xu, Si Wu, Alexander K. Buell, Samuel I. A. Cohen, Li-Jun Chen, Wan-Hui Hu, Sarah A. Cusack, Laura S. Itzhaki, Hong Zhang, Tuomas P. J. Knowles, Christopher M. Dobson, Mark E. Welland, Gary W. Jones and Sarah Perrett Influence of specific HSP70 domains on fibril formation of the yeast prion protein Ure2 Philos Trans R Soc Lond B Biol Sci 368, 20110410 (2013) http://rstb.royalsocietypublishing.org/content/368/1617/20110410.long
Tim Guilliams, Farah El-Turk, Alexander K. Buell, Elizabeth O'Day, Francesco Aprile, Elin K. Esbjörner, Michele Vendruscolo, Nunilo Cremades, Els Pardon, Lode Wyns, Mark E. Welland, Jan Steyaert, John Christodoulou, Christopher M. Dobson and Erwin De Genst Nanobodies raised against monomeric α-synuclein distinguish between fibrils at different maturation stages J Mol Biol 425, 2397-2411 (2013) http://www.sciencedirect.com/science/article/pii/S0022283613001897
Alexander K. Buell, Christopher M. Dobson, Mark E. Welland Measuring the kinetics of amyloid fibril elongation using Quartz Crystal Microbalances (QCM) Amyloid proteins - Methods and Protocols 849, 101-119 (2012) http://link.springer.com/protocol/10.1007%2F978-1-61779-551-0_8
Cintia Roodveldt, August Andersson, Erwin J. De Genst, Adahir Labrador-Garrido, Alexander K. Buell, Christopher M. Dobson, Gian Gaetano Tartaglia and Michele Vendruscolo A Rationally Designed Six-Residue Swap Generates Comparability in the Aggregation Behavior of α-Synuclein and β-Synuclein Biochemistry 51, 8771-8778 (2012) http://pubs.acs.org/doi/abs/10.1021/bi300558q
Alexander K. Buell, Anne Dhulesia (joint first authors), Maria F. Mossuto, Nunilo Cremades, Janet R. Kumita, Mireille Dumoulin, Mark E. Welland, Tuomas P. J. Knowles, Xavier Salvatella, Christopher M. Dobson Population of non-native states of lysozyme mutants drives amyloid fibril formation JACS 133, 7737-7743 (2011) http://pubs.acs.org/doi/abs/10.1021/ja109620d
Alexander K. Buell, Elin K. Esbjörner, Patrick J. Riss, Duncan A. White, Franklin I. Aigbirhio, Gergely Toth, Mark E. Welland, Christopher M. Dobson and Tuomas P.J. Knowles Probing small molecule binding to amyloid fibrils Phys Chem Chem Phys 13, 20044-20052 (2011) https://www.ncbi.nlm.nih.gov/pubmed/22006124
Sarah L. Shammas, Christopher A. Waudby, Shuyu Wang, Alexander K. Buell, Tuomas P.J. Knowles, Heath Ecroyd, Mark E. Welland, John A. Carver, Christopher M. Dobson and Sarah Meehan Binding of the Molecular Chaperone alpha B-Crystallin to Abeta Amyloid Fibrils Inhibits Fibril Elongation Biophysical Journal 101, 1681-1689 (2011) https://www.ncbi.nlm.nih.gov/pubmed/21961594
Yi-Qian Wang, Alexander K. Buell, Xin-Yu Wang, Mark E. Welland, Christopher M. Dobson, Tuomas P.J Knowles, Sarah Perrett Relationship Between Prion Propensity and the Rates of Individual Molecular Steps of Fibril Assembly J Biol Chem 286, 12101-12107 (2011) http://www.jbc.org/content/286/14/12101.long
Alexander K. Buell, Christopher M. Dobson, Tuomas P.J. Knowles and Mark E. Welland Interactions between amyloidophilic dyes and their relevance to studies of amyloid inhibitors Biophysical Journal 99(10), 3492-3497 (2010) http://www.cell.com/biophysj/references/S0006-3495(10)01108-2
Alexander K. Buell, Duncan A. White, Christoph Meier, Mark E. Welland, Tuomas P.J. Knowles and Christopher M. Dobson Surface attachment of protein fibrils via covalent modication strategies J Phys Chem B 114, 10925-10938 (2010) http://pubs.acs.org/doi/abs/10.1021/jp101579n
Alexander K. Buell, Jamie R. Blundell, Christopher M. Dobson, Mark E. Welland, Eugene M. Terentjev and Tuomas P. J. Knowles Frequency factors in a landscape model of filamentous protein aggregation Physical Review Letters 104, 228101 (2010) http://journals.aps.org/prl/abstract/10.1103/PhysRevLett.104.228101
Duncan A. White, Alexander K. Buell, Tuomas P.J. Knowles, Mark E. Welland and Christopher M. Dobson Protein aggregation in crowded environments JACS 132(14), 5170-5175 (2010) http://pubs.acs.org/doi/abs/10.1021/ja909997e
Tuomas P. J. Knowles, Tomas W. Oppenheim, Alexander K. Buell, Dimitri Y. Chirgadze and Mark E. Welland Nanostructured films from hierarchical self-assembly of amyloidogenic proteins Nature Nanotechnology 5, 204-207 (2010) http://www.nature.com/nnano/journal/v5/n3/abs/nnano.2010.26.html
Alexander K. Buell, Gian Gaetano Tartaglia, Neil R. Birkett, Christopher A. Waudby, Michele Vendruscolo, Xavier Salvatella, Mark E. Welland, Christopher M. Dobson and Tuomas P.J. Knowles Position dependent electrostatic protection against protein aggregation ChemBioChem 10(8), 1309-1312 (2009) http://onlinelibrary.wiley.com/doi/10.1002/cbic.200900144/abstract
W. Scott Hopkins, Hans-Peter Loock, Brid Cronin, Michael G. D. Nix, Adam L. Devine, Richard N. Dixon, Michael N. R. Ashfold, Hong-Ming Yin, Steven J. Rowling, Alexander Büll and Scott H. Kable Quantitative (v, N, Ka ) product state distributions near the triplet threshold for the reaction H2CO → H + HCO measured by Rydberg tagging and laser-induced fluorescence J Phys Chem A 112(39), 9283-9289 (2008) http://pubs.acs.org/doi/abs/10.1021/jp8021826?src=recsys&journalCode=jpcafh
Hong-Ming Yin, Steven J. Rowling, Alexander Büll and Scott H. Kable Photodissociation dynamics of the reaction H2CO → H+HCO via the singlet (S0) and triplet (T1) surfaces J Chem Phys 127(6), 064302 (2007) http://aip.scitation.org/doi/abs/10.1063/1.2752161?journalCode=jcp