Florian Bleffert, Joachim Granzin, Muttalip Caliskan, Stephan N Schott-Verdugo, Meike Siebers, Björn Thiele, Laurence Rahme, Sebastian Felgner, Peter Dörmann, Holger Gohlke* , Renu Batra-Safferling*, Karl-Erich Jaeger, Filip Kovacic* Structural, mechanistic, and physiological insights into phospholipase A-mediated membrane phospholipid degradation in Pseudomonas aeruginosa. eLife 11, e72824 (2022) https://doi.org/10.7554/eLife.72824
Hemmer S, Schulte M, Knieps-Grünhagen E, Granzin J, Willbold D, Jaeger KE, Batra-Safferling R, Panwalkar V, Krauss U. Residue alterations within a conserved hydrophobic pocket influence light, oxygen, voltage photoreceptor dark recovery. Photochem Photobiol Sci , (2022) https://doi.org/10.1007/s43630-022-00346-5
Weiergräber OH, Petrović D, Kislat A, Pattky M, Fabig J, Batra-Safferling R, Schulte am Esch J, Hänel K, Huhn C, Strodel B, Homey B, Willbold D Structure and Dynamics of Human Chemokine CCL16—Implications for Biological Activity Biomolecules 12, 1588 (2022) https://doi.org/10.3390/biom12111588
Arinkin V, Granzin J, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R. Structural determinants underlying the adduct lifetime in the LOV proteins of Pseudomonas putida. FEBS J 288(16), 4955-4972 (2021) https://doi.org/10.1111/febs.15785
Röllen K, Granzin J, Remeeva A, Davari MD, Gensch T, Nazarenko VV, Kovalev K, Bogorodskiy A, Borshchevskiy V, Hemmer S, Schwaneberg U, Gordeliy V, Jaeger KE, Batra-Safferling R, Gushchin I, Krauss U. The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins. J Biol Chem 296, 100662 (2021) https://doi.org/10.1016/j.jbc.2021.100662
Stadler AM, Granzin J, Cousin A, Batra-Safferling R. Phosphorylated peptide of G protein-coupled receptor induces dimerization in activated arrestin. Scientific Reports 10, 10938 (2020) https://doi.org/10.1038/s41598-020-67944-0
Fettweiss T, Röllen K, Granzin J, Reiners O, Endres S, Drepper T, Willbold D, Jaeger KE, Batra-Safferling R*, Krauss U* Mechanistic Basis of the Fast Dark Recovery of the Short LOV Protein DsLOV from Dinoroseobacter shibae. Biochemistry 57, 4833–4847 (2018) http://dx.doi.org/10.1021/acs.biochem.8b00645
Röllen, K.; Granzin, J.; Batra-Safferling, R*.; Stadler, A.M*. Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein PLoS One 13(7):e0200746, (2018) http://dx.doi.org/10.1371/journal.pone.0200746
Arinkin V, Granzin J, Röllen K, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R. Structure of a LOV protein in apo-state and implications for construction of LOV-based optical tools Scientific Reports 7(42971), (2017) http://dx.doi.org/10.1038/srep42971
Batra-Safferling R and Granzin J. The Structure of the Polar Core Mutant R175E and Its Functional Implications. The Structural Basis Of Arrestin Functions, Vsevolod V. Gurevich (Editor); Springer International Publishing Chapter 11, 143-158 (2017) ISBN: 978-3-319-57552-0
Schlesinger R, Cousin A, Granzin J, Batra-Safferling R. Expression and purification of arrestin in yeast Saccharomyces cerevisiae. Methods Cell Biol 142, 159-172 (2017) https://doi.org/10.1016/bs.mcb.2017.07.003
Röllen K, Granzin J, Panwalkar V, Arinkin V, Rani R, Hartmann R, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R. Signaling States of a Short Blue-Light Photoreceptor Protein PpSB1-LOV Revealed from Crystal Structures and Solution NMR Spectroscopy. J Mol Biol. 428, 3721 - 3736 (2016) http://dx.doi.org/10.1016/j.jmb.2016.05.027 http://www.ncbi.nlm.nih.gov/pubmed/27291287
Endres S, Granzin J, Circolone F, Stadler A, Krauss U, Drepper T, Svensson V, Knieps-Grünhagen E, Wirtz A, Cousin A, Tielen P, Willbold D, Jaeger K-E, Batra-Safferling R Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae. BMC Microbiology 15, 30 (2015) http://www.biomedcentral.com/1471-2180/15/30
Circolone F, Granzin J, Jentzsch K, Drepper T, Jaeger K-E, Willbold D, Krauss U, Batra-Safferling R Structural basis for the slow dark recovery of a full-length LOV protein from Pseudomonas putida. J. Mol. Biol. 417, 362–374 (2012)
Granzin J, Cousin A, Weirauch M, Schlesinger R, Büldt G, Batra-Safferling R Crystal structure of p44, a constitutively active splice variant of visual arrestin. J Mol Biol. 416(5), 611-8 (2012) http://dx.doi.org/10.1016/j.jmb.2012.01.028
Granzin J, Huang Y, Topbas C, Huang W, Wu Z, Misra S, Hazen SL, Blanton RE, Lee X, Weiergräber OH Three-dimensional structure of a schistosome serpin revealing an unusual configuration of the helical subdomain Acta Cryst D68, 686-694 (2012) http://dx.doi.org/10.1107/S0907444912008372
Kirchberg K, Kim TY, Möller M, Skegro D, Dasara Raju G, Granzin J, Büldt G, Schlesinger R, Alexiev U Conformational dynamics of helix 8 in the GPCR rhodopsin controls arrestin activation in the desensitization process. Proc Natl Acad Sci U S A 108(46), 18690-5 (2011) http://dx.doi.org/10.1073/pnas.1015461108
Batra-Safferling R, Granzin J, Moedder S, Hoffmann S, Willbold D Structural studies of PI3K SH3 domain in complex with a peptide ligand: Role of anchor residue in ligand binding. Biol. Chem. 391, 33-42 (2010)
Feuerstein SE, Pulvermüller A, Hartmann R, Granzin J, Stoldt M, Henklein P, Ernst OP, Heck M, Willbold D, Koenig BW Helix formation in Arrestin accompanies recognition of photoactivated Rhodopsin. Biochemsitry 48, 10733-42 (2009)
Skegro D, Pulvermüller A, Krafft B, Granzin J, Hofmann KP, Büldt G, Schlesinger R N-terminal and C-terminal domains of arrestin both contribute in binding to rhodopsin. Photochem Photobiol. 83(2), 385-92 (2007)
Batra-Safferling R, Abaraca-Heidemann K, Körschen HG, Tziatzios C, Stoldt M, Budyak I, Willbold D, Schwalbe H, Klein-Seetharaman J, Kaupp UB Glutamic acid-rich proteins of rod photoreceptors are natively unfolded. J Biol Chem 281, 1449-1460 (2006)
Granzin J, Eckhoff A, Weiergräber OH Crystal structure of a multi-domain immunophilin from Arabidopsis thaliana: a paradigm for regulation of plant ABC transporters J Mol Biol 364, 799-809 (2006)
Weiergräber OH, Eckhoff A, Granzin J Crystal structure of a plant immunophilin domain involved in regulation of MDR-type ABC transporters FEBS Lett 580, 251-255 (2006)
Weiergräber OH, Senin II, Zernii EY, Churumova VA, Kovaleva NA, Nazipova AA, Permyakov SE, Permyakov EA, Philippov PP, Granzin J, Koch KW Tuning of a neuronal calcium sensor J Biol Chem 281, 37594-37602 (2006)
Eckhoff A, Granzin J, Kamphausen T, Büldt G, Schulz B, Weiergräber OH Crystallization and preliminary X-ray analysis of immunophilin-like FKBP42 from Arabidopsis thaliana Acta Crystallogr F 61, 363-365 (2005)
Khayat R, Batra R, Bebernitz GA, Olson MW, Tong L Characterization of the monomer-dimer equilibrium of human cytomegalovirus protease by kinetic methods. Biochemistry 43(2), 316-22 (2004)
Choe HW, Park KS, Labahn J, Granzin J, Kim CJ, Büldt G Crystallization and preliminary X-ray diffraction studies of alpha-cyclodextrin glucanotransferase isolated from Bacillus macerans. Acta Crystallogr D Biol Crystallogr. 59(Pt 2), 348-9 (2003)
Khayat R, Batra R, Qian C, Halmos T, Bailey M, Tong L Structural and biochemical studies of inhibitor binding to human cytomegalovirus protease. Biochemistry 42(4), 885-91 (2003)
Weiergräber OH, Senin II, Philippov PP, Granzin J, Koch KW Impact of N-terminal myristoylation on the Ca2+-dependent conformational transition in recoverin J Biol Chem 278, 22972-22979 (2003)
Batra R, Christendat D, Edwards A, Arrowsmith C, Tong L Crystal structure of MTH169, a crucial component of phosphoribosylformylglycinamidine synthetase. Proteins 49(2), 285-8 (2002)
Labahn J, Neumann S, Büldt G, Kula MR, Granzin J An alternative mechanism for amidase signature enzymes. J Mol Biol. 322(5), 1053-64 (2002)
Neumann S, Granzin J, Kula MR, Labahn J Crystallization and preliminary X-ray data of the recombinant peptide amidase from Stenotrophomonas maltophilia. Acta Crystallogr D Biol Crystallogr. 58(Pt 2), 333-5 (2002)
Tsiavaliaris G, Fujita-Becker S, Batra R, Levitsky DI, Kull FJ, Geeves MA, Manstein DJ Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium. EMBO Rep. 3(11), 1099-105 (2002)
Batra R, Khayat R, Tong L Molecular mechanism for dimerization to regulate the catalytic activity of human cytomegalovirus protease. Nature Struct Biol. 8(9), 810-7 (2001)
Khayat R, Batra R, Massariol MJ, Lagacé L, Tong L Investigating the role of histidine 157 in the catalytic activity of human cytomegalovirus protease. Biochemistry 40(21), 6344-51 (2001)
Yang Z, Batra R, Floyd DL, Hung HC, Chang GG, Tong L Potent and competitive inhibition of malic enzymes by lanthanide ions. Biochem Biophys Res Commun 2;274(2), 440-4 (2000)
Batra R, Geeves MA, Manstein DJ Kinetic analysis of Dictyostelium discoideum myosin motor domains with glycine-to-alanine mutations in the reactive thiol region. Biochemistry 38(19), 6126-34 (1999)
Batra R, Manstein DJ Functional characterisation of Dictyostelium myosin II with conserved tryptophanyl residue 501 mutated to tyrosine Biol Chem. 380(7-8), 1017-23 (1999)
Van Dijk J, Furch M, Derancourt J, Batra R, Knetsch ML, Manstein DJ, Chaussepied P Differences in the ionic interaction of actin with the motor domains of nonmuscle and muscle myosin II. Eur J Biochem 260(3), 672-83 (1999)
Granzin J, Wilden U, Choe HW, Labahn J, Krafft B, Büldt G X-ray crystal structure of arrestin from bovine rod outer segments. Nature 391(6670), 918-21 (1998)
Schluckebier G, Labahn J, Granzin J, Saenger W M.TaqI: possible catalysis via cation-pi interactions in N-specific DNA methyltransferases. Biol Chem. 379(4-5), 389-400 (1998)
Batra R, Tyagi R, Gupta MN Influence of immobilization on enzyme activity in aqueous-organic cosolvent mixtures Biocatalysis and Biotransformation 15(2), 101-119 (1997)
Gupta MN, Batra R, Tyagi R, Sharma, A Polarity index: The guiding solvent parameter for enzyme stability in aqueous-organic cosolvent mixtures Biotechnology Progress 13(3), 284-288 (1997)
Wilden U, Choe HW, Krafft B, Granzin J Crystallization and preliminary X-ray analysis of arrestin from bovine rod outer segment. FEBS Lett. 415(3), 268-70 (1997)
Dong GQ, Batra R, Kaul R, Gupta MN, Mattiasson B Alternative modes of precipitation of Eudragit S100: A potential ligand carrier for affinity precipitation of protein Bioseparation 5(6), 339-350 (1995)
Schluckebier G, Labahn J, Granzin J, Schildkraut I, Saenger W A model for DNA binding and enzyme action derived from crystallographic studies of the TaqI N6-adenine-methyltransferase. Gene 157(1-2), 131-4 (1995)
Batra R, Gupta MN Enhancement of Enzyme-Activity in Aqueous-Organic Solvent Mixtures Biotechnology Letters 16(10), 1059-1064 (1994)
Batra R, Gupta MN Noncovalent Immobilization of Potato (Solanum-Tuberosum) Polyphenol Oxidase on Chitin Biotechnology and Applied Biochemistry 19, 209-215 (1994)
Cvak L, Jegorov A, Sedmera P, Havlicek V, Ondracek J, Husak M, Pakhomova S, Kratochvil B, Granzin J Ergogaline, a New Ergot Alkaloid, Produced by Claviceps purpurea: Isolation, Identification, Crystal Structure and Molecular conformation. J. Chem. Soc. PERKIN TRANS. 2, 1861-1865 (1994)
Kumar A, Agarwal R, Batra R, Gupta MN Effect of Polymer Concentration on Recovery of the Target Proteins in Precipitation Methods Biotechnology Techniques 8(9), 651-654 (1994)
Labahn J, Granzin J, Schluckebier G, Robinson DP, Jack WE, Schildkraut I, Saenger W Three-dimensional structure of the adenine-specific DNA methyltransferase M.Taq I in complex with the cofactor S-adenosylmethionine. Proc Natl Acad Sci U S A. 91(23), 10957-61 (1994)
Schubert WD, Schluckebier G, Backmann J, Granzin J, Kisker C, Choe HW, Hahn U, Pfeil W, Saenger W X-ray crystallographic and calorimetric studies of the effects of the mutation Trp59-->Tyr in ribonuclease T1. Eur J Biochem. 220(2), 527-34 (1994)
Lenz A, Choe HW, Granzin J, Heinemann U, Saenger W Three-dimensional structure of the ternary complex between ribonuclease T1, guanosine 3',5'-bisphosphate and inorganic phosphate at 0.19 nm resolution. Eur J Biochem. 211(1-2), 311-6 (1993)
Saenger W, Sandmann C, Theis K, Starikov EB, Kostrewa D, Labahn J, Granzin J Structural and Functions Aspects of the DNA Binding Protein FIS; for Nucleic Acids and Molecular Biology. Edited by Fritz Eckstein and David M.J. Lilley. Nucleic Acids and Molecular Biology Vol 7, Springer-Verlag. , (1993)
Ding J, Choe HW, Granzin J, Saenger W Structure of ribonuclease T1 complexed with zinc(II) at 1.8 A resolution: a Zn2+.6H2O.carboxylate clathrate. Acta Crystallogr B. 48 ( Pt 2), 185-91 (1992)
Granzin J, Puras-Lutzke R, Landt O, Grunert HP, Heinemann U, Saenger W, Hahn U RNase T1 mutant Glu46Gln binds the inhibitors 2'GMP and 2'AMP at the 3' subsite. J Mol Biol. 225(2), 533-42. (1992)
Kostrewa D, Granzin J, Koch C, Choe HW, Raghunathan S, Wolf W, Labahn J, Kahmann R, Saenger W Three-Dimensional Structure of The DNA Binding Protein FIS. Structure & Function Vol. 2: Proteins. Eds. R.H. Sarma & M.H. Sarma, Adenine Press. , (1992)
Kostrewa D, Granzin J, Stock D, Choe HW, Labahn J, Saenger W Crystal structure of the factor for inversion stimulation FIS at 2.0 A resolution. J Mol Biol. 226(1), 209-26 (1992)
Keitel T, Granzin J, Simon O, Borriss R, Thomsen KK, Wessner H, Höhne W, Heinemann U Crystallization of the hybrid Bacillus (1-3,1-4)-beta-glucanase H(A16-M). J Mol Biol. 218(4), 703-4 (1991)
Kostrewa D, Granzin J, Koch C, Choe HW, Raghunathan S, Wolf W, Labahn J, Kahmann R, Saenger W Three-dimensional structure of the E. coli DNA-binding protein FIS. Nature 349(6305), 178-80 (1991)
Granzin J, Saenger W, Stolarski R, Shugar D Solid State and Solution Structures of an Adenine Analogue of the Antiviral Acyclonucleoside 9- (1,3-Dihydroxy-2-propoxymethyl) guanine. Z. Naturforsch 45c, 915-921 (1990)
Weber H-J, Schulz M, Schmitz S, Granzin J, Siegert H Determination and structural application of anisotropic bond polarisabilities in complex crystals. J. Phys.: Condens. Matter 1, 8543-8557 (1989)
Witt I, Witt HT, Di Fiore D, Röger M, Hinrichs W, Saenger W, Granzin J, Betzel C, Dauter Z X-ray characterization of Single Crystals of the Reaction Center I of Water Splitting Photosysnthesis. Ber. Bunsenges. Phys. Chem. 92, 1503-1506 (1988)
